Tested Applications
| Positive IHC detected in | mouse brain tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
19776-1-AP targets ADRA1B-Specific in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | adrenergic, alpha-1B-, receptor |
| Calculated Molecular Weight | 57 kDa |
| GenBank Accession Number | NM_000679 |
| Gene Symbol | ADRA1B |
| Gene ID (NCBI) | 147 |
| RRID | AB_2848149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35368 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADRA1B belongs to the G-protein coupled receptor 1 family. ADRA1B mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This antibody is specific to ADRA1B.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADRA1B-Specific antibody 19776-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Andrea (Verified Customer) (07-31-2023) | Good signal in flow cytometry. Endogenously expressed protein and confirmed with overexpressing cell line
|
FH Mohamad (Verified Customer) (07-03-2023) | different bands at different MW
|
