AFM Polyclonal antibody

AFM Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 25280-1-AP

Synonyms

afamin, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin



Tested Applications

Positive WB detected inhuman blood tissue
Positive IP detected inhuman plasma tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25280-1-AP is AFM Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFM fusion protein Ag16866
Full Name afamin
Calculated molecular weight 599 aa, 69 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC109020
Gene symbol AFM
Gene ID (NCBI) 173
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

Protocols

Product Specific Protocols
WB protocol for AFM antibody 25280-1-APDownload protocol
IP protocol for AFM antibody 25280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols