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AFM Polyclonal antibody

AFM Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25280-1-AP

Synonyms

afamin, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin

Validation Data Gallery

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  • Western Blot (WB) analysis of human blood using AFM Polyclonal antibody (25280-1-AP)

    WB analysis of human blood using 25280-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 25280-1-AP (AFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using AFM Polyclonal antibody (25280-1-AP)

    IP experiment of human plasma using 25280-1-AP

    IP result of anti-AFM (IP:25280-1-AP, 4ug; Detection:25280-1-AP 1:2000) with human plasma lysate 4000ug.

Tested Applications

Positive WB detected inhuman blood tissue
Positive IP detected inhuman plasma tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25280-1-AP is AFM Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFM fusion protein Ag16866
Full Name afamin
Calculated Molecular Weight 599 aa, 69 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC109020
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2880005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.

Protocols

Product Specific Protocols
WB protocol for AFM antibody 25280-1-APDownload protocol
IP protocol for AFM antibody 25280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols