Tested Applications
| Positive WB detected in | MCF-7 cells, HepG2 cells, mouse testis tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
83442-6-RR targets AGA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag10681 Product name: Recombinant human AGA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC012392 Sequence: MARKSNLPVLLVPFLLCQALVRCSSPLPLVVNTWPFKNATEAAWRALASGGSALDAVESGCAMCEREQCDGSVGFGGSPDELGETTLDAMIMDGTTMDVGAVGDLRRIKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTSASQALHSDWLARNCQPNYWRNVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRGHDTIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACNKLSTFTQFSFMVYNSEKNQPTEEKVDCI Predict reactive species |
| Full Name | aspartylglucosaminidase |
| Calculated Molecular Weight | 346 aa, 37 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC012392 |
| Gene Symbol | AGA |
| Gene ID (NCBI) | 175 |
| RRID | AB_3671083 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P20933 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AGA(Aspartylglucosaminidase) is also named as N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase and glycosylasparaginase.The open reading frame of the readthrough transcript would indicate AGA close to 40 kDa. It can be cleaved into 2 chains:the 24 kDa α- and 17 kDa β-subunits of the wild-type AGA,in addition,an aberrant polypeptide with a mol.wt of 21 kDa is also detected as a product of proteolytic cleavage of the mutant precursor molecule(PMID: 9425233).AGA can exsit as a dimer with the mol.wt between 100 kDa and 49 kDa and His124 at an interface between two heterodimers of AGA is crucial for the thermodynamically stable oligomeric structure of AGA.(PMID:9737998). It can also exsit as a heterotetrameric protein of 88 kDa(PMID:1733831).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AGA antibody 83442-6-RR | Download protocol |
| IHC protocol for AGA antibody 83442-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
