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AGFG1 Polyclonal antibody

AGFG1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12670-1-AP

Synonyms

AGFG1, ArfGAP with FG repeats 1, HIV 1 Rev binding protein, HRB, Nucleoporin like protein RIP, RAB, Rev interacting protein, RIP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using AGFG1 Polyclonal antibody (12670-1-AP)

    WB analysis of mouse testis using 12670-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using AGFG1 Polyclonal antibody (12670-1-AP)

    WB analysis of K-562 using 12670-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AGFG1 Polyclonal antibody (12670-1-AP)

    WB analysis of HeLa using 12670-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using AGFG1 Polyclonal antibody (12670-1-AP)

    WB analysis of Jurkat using 12670-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using AGFG1 Polyclonal antibody (12670-1-AP)

    WB analysis of HT-1080 using 12670-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AGFG1 Polyclonal antibody (12670-1-AP)

    WB analysis of HepG2 using 12670-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12670-1-AP (AGFG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using AGFG1 Polyclonal antibody (12670-1-AP)

    IP experiment of mouse testis using 12670-1-AP

    IP result of anti-AGFG1 (IP:12670-1-AP, 4ug; Detection:12670-1-AP 1:400) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AGFG1 Polyclonal antibody (12670-1-AP)

    IHC staining of human breast cancer using 12670-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12670-1-AP (AGFG1 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells, HepG2 cells, HT-1080 cells, Jurkat cells, K-562 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12670-1-AP targets AGFG1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGFG1 fusion protein Ag3352
Full Name ArfGAP with FG repeats 1
Calculated Molecular Weight 562 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC030592
Gene Symbol AGFG1
Gene ID (NCBI) 3267
RRIDAB_2258077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AGFG1 antibody 12670-1-APDownload protocol
IHC protocol for AGFG1 antibody 12670-1-APDownload protocol
IP protocol for AGFG1 antibody 12670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell

A Translation-Activating Function of MIWI/piRNA during Mouse Spermiogenesis.

Authors - Peng Dai
View Article

Proc Natl Acad Sci U S A

Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse.

Authors - Yoshinori Kanemori
View Article