• Featured Product
  • KD/KO Validated

AHCY Polyclonal antibody

AHCY Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10757-2-AP

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH



Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, HepG2 cells, mouse thymus tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10757-2-AP targets AHCY in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Amphioxus, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHCY fusion protein Ag1208
Full Name S-adenosylhomocysteine hydrolase
Calculated molecular weight 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC011606
Gene symbol AHCY
Gene ID (NCBI) 191
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Protocols

Product Specific Protocols
WB protocol for AHCY antibody 10757-2-APDownload protocol
IHC protocol for AHCY antibody 10757-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Longev Healthspan

Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice.

Authors - Holly M Brown-Borg
human,mouseWB

Toxicol Sci

Identification of Serum Biomarkers to Distinguish Hazardous and Benign Aminotransferase Elevations.

Authors - Joel H Vazquez
humanWB

Cell Stem Cell

Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.

Authors - Rashim Pal Singh
AmphioxusWB,IHC

Biosci Rep

Characterization, expression and localization of S-adenosylhomocysteine hydrolase from amphioxus Branchiostoma belcheri tsingtaunese.

Authors - Wang Yuan Y
ratWB

Environ Toxicol Pharmacol

Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium.

Authors - Wenhua Kuang
humanWB

Mol Biol Rep

Overexpression of S-adenosylhomocysteine hydrolase (SAHH) in esophageal squamous cell carcinoma (ESCC) cell lines: effects on apoptosis, migration and adhesion of cells.

Authors - Qinghua Li