Tested Applications
| Positive WB detected in | HepG2 cells, COLO 320 cells, HeLa cells, mouse liver tissue, Jurkat cells, rat liver tissue |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10757-2-AP targets AHCY in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, branchiostoma belcheri |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1208 Product name: Recombinant human AHCY protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 116-432 aa of BC011606 Sequence: QTLYFKDGPLNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY Predict reactive species |
| Full Name | S-adenosylhomocysteine hydrolase |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 45-48 kDa |
| GenBank Accession Number | BC011606 |
| Gene Symbol | AHCY |
| Gene ID (NCBI) | 191 |
| RRID | AB_2289488 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23526 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AHCY antibody 10757-2-AP | Download protocol |
| IP protocol for AHCY antibody 10757-2-AP | Download protocol |
| WB protocol for AHCY antibody 10757-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-AHCY antibody (Cat# 10757-2-AP) has 33 citations published in journals including Nature Metabolism, Nature Communications, Cell Metabolism, Cell Stem Cell, Autophagy, Clinical Cancer Research, Oncogene, iScience, and Journal of Biological Chemistry. Associated research fields encompass methionine/one-carbon metabolism, DNA methylation and epigenetics, cancer (colorectal, esophageal, glioblastoma, AML), autophagy, ferroptosis, cardiovascular disease, liver disease, stem cell biology, bone differentiation, reproductive biology, and environmental toxicology.
| Species | Application | Title |
|---|---|---|
Exploration (Beijing) STIP1 drives Metabolic Reprogramming in Esophageal Squamous Cell Carcinoma via AHCY-LDHA Axis.
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Nat Metab Metabolic profiling stratifies colorectal cancer and reveals adenosylhomocysteinase as a therapeutic target | ||
Cell Stem Cell Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal. | ||
Autophagy AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner.
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Nat Commun H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.
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Reviews
What customers say
The single review for 10757-2-AP is a 5-star rating from January 2024. The user performed a Western Blot on mouse liver tissue using a 1:5000 dilution (Lot 00044560) and reported that the antibody worked well at the recommended dilution when diluted with 1X TBST. Overall, the feedback is positive, confirming reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kamal (Verified Customer) (01-26-2024) | Antibody worked well for western blot analysis when diluted with 1X TBST at the provided dilution.
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