|Positive WB detected in||mouse liver tissue, HeLa cells, HepG2 cells, mouse thymus tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:300-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10757-2-AP targets AHCY in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Amphioxus, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AHCY fusion protein Ag1208|
|Full Name||S-adenosylhomocysteine hydrolase|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||45-48 kDa|
|GenBank accession number||BC011606|
|Gene ID (NCBI)||191|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).
Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice.
Identification of Serum Biomarkers to Distinguish Hazardous and Benign Aminotransferase Elevations.
Cell Stem Cell
Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.
Characterization, expression and localization of S-adenosylhomocysteine hydrolase from amphioxus Branchiostoma belcheri tsingtaunese.
Environ Toxicol Pharmacol
Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium.
Mol Biol Rep
Overexpression of S-adenosylhomocysteine hydrolase (SAHH) in esophageal squamous cell carcinoma (ESCC) cell lines: effects on apoptosis, migration and adhesion of cells.