Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, mouse thymus tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10658-3-AP targets AHCYL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1017 Product name: Recombinant human AHCYL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 212-530 aa of BC007576 Sequence: IDRCVNMDGWQANMILDDGGDLTHWVYKKYPNVFKKIRGIVEESVTGVHRLYQLSKAGKLCVPAMNVNDSVTKQKFDNLYCCRESILDGLKRTTDVMFGGKQVVVCGYGEVGKGCCAALKALGAIVYITEIDPICALQACMDGFRVVKLNEVIRQVDVVITCTGNKNVVTREHLDRMKNSCIVCNMGHSNTEIDVTSLRTPELTWERVRSQVDHVIWPDGKRVVLLAEGRLLNLSCSTVPTFVLSITATTQALALIELYNAPEGRYKQDVYLLPKKMDEYVASLHLPSFDAHLTELTDDQAKYLGLNKNGPFKPNYYRY Predict reactive species |
| Full Name | S-adenosylhomocysteine hydrolase-like 1 |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC007576 |
| Gene Symbol | AHCYL1 |
| Gene ID (NCBI) | 10768 |
| RRID | AB_2226013 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43865 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor-binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release into the cytoplasm of cells(PMID:23145124). This protein is also named as DCAL, XPVKONA and belongs to the adenosylhomocysteinase family. AHCYL1 mRNA and protein are most abundant in the glandular epithelium of adenocarcinoma of cancerous, but not normal and AHCYL1 protein is predominantly in the nucleus(PMID:22826361). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AHCYL1 antibody 10658-3-AP | Download protocol |
| IHC protocol for AHCYL1 antibody 10658-3-AP | Download protocol |
| IP protocol for AHCYL1 antibody 10658-3-AP | Download protocol |
| WB protocol for AHCYL1 antibody 10658-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
AHCYL1 Polyclonal antibody (Cat# 10658-3-AP) has 23 citations published in journals including Molecular Cell, Cell Death & Differentiation, Journal of Agricultural and Food Chemistry, Molecular Nutrition & Food Research, Poultry Science, Nutrients, Journal of Gastroenterology, PLoS ONE, Neuroscience, and Anticancer Research. Research fields span epigenetics/DNA methylation, lipid and cholesterol metabolism, ferroptosis, cancer, pancreatitis, spinal cord injury, and neurodevelopment.
| Species | Application | Title |
|---|---|---|
Cell Death Differ Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation | ||
J Anim Sci Biotechnol Betaine alleviates hepatic lipid metabolism disorder in finishing pigs fed a low-energy diet through regulating m(6)A RNA methylation. | ||
Pharmaceutics Protective Role of IRBIT on Sodium Bicarbonate Cotransporter-n1 for Migratory Cancer Cells. | ||
J Agric Food Chem Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets. | ||
J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. |
