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AHCYL1 Polyclonal antibody

AHCYL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10658-3-AP

Synonyms

AdoHcyase 2, AHCYL1, DCAL, IRBIT, PRO0233, XPVKONA



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse thymus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10658-3-AP targets AHCYL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, Landrace, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHCYL1 fusion protein Ag1017
Full Name S-adenosylhomocysteine hydrolase-like 1
Calculated molecular weight 65 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC007576
Gene symbol AHCYL1
Gene ID (NCBI) 10768
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor-binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release into the cytoplasm of cells(PMID:23145124). This protein is also named as DCAL, XPVKONA and belongs to the adenosylhomocysteinase family. AHCYL1 mRNA and protein are most abundant in the glandular epithelium of adenocarcinoma of cancerous, but not normal and AHCYL1 protein is predominantly in the nucleus(PMID:22826361). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AHCYL1 antibody 10658-3-APDownload protocol
IHC protocol for AHCYL1 antibody 10658-3-APDownload protocol
IF protocol for AHCYL1 antibody 10658-3-APDownload protocol
IP protocol for AHCYL1 antibody 10658-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

J Agric Food Chem

Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.

Authors - Yimin Jia
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
chickenWB

Comp Biochem Physiol B Biochem Mol Biol

Maternal betaine administration modulates hepatic type 1 iodothyronine deiodinase (Dio1) expression in chicken offspring through epigenetic modifications.

Authors - Zhen Hou
humanIHC

Anticancer Res

Low IRBIT Levels Are Associated With Chemo-resistance in Gastric Cancer Patients.

Authors - Nobuhiro Nakazawa
chickenWB

Poult Sci

Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes.

Authors - Yun Hu
pigWB

J Agric Food Chem

Down-regulation of miR-150 expression by DNA hypermethylation is associated with high HMB-induced hepatic cholesterol accumulation in nursery piglets.

Authors - Yimin Jia