AKT3 Polyclonal antibody

AKT3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21641-1-AP

Synonyms

AKT3, PKB GAMMA, PKBG, PRKBG, Protein kinase Akt 3, Protein kinase B gamma, RAC gamma, RAC PK gamma, STK 2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

The immunogen of 21641-1-AP is AKT3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKT3 fusion protein Ag16298
Full Name v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Calculated molecular weight 465 aa, 54 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC121154
Gene symbol AKT3
Gene ID (NCBI) 10000
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKT3, also named as PKBG, is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas.

Protocols

Product Specific Protocols
WB protocol for AKT3 antibody 21641-1-APDownload protocol
IHC protocol for AKT3 antibody 21641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

EGR1 interacts with DNMT3L to inhibit the transcription of miR-195 and plays an anti-apoptotic role in the development of gastric cancer.

Authors - Yang Yang
humanWB

Front Oncol

Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer.

Authors - Yi-Yuan Sun
humanWB

Cell Immunol

MicroRNA-150 negatively regulates the function of CD4(+) T cells through AKT3/Bim signaling pathway.

Authors - Wei Sang

Oncol Rep

MYO6 knockdown inhibits the growth and induces the apoptosis of prostate cancer cells by decreasing the phosphorylation of ERK1/2 and PRAS40.

Authors - Dong Wang
humanWB

J Ovarian Res

Role of BMI1 in epithelial ovarian cancer: investigated via the CRISPR/Cas9 system and RNA sequencing.

Authors - Qianying Zhao
humanWB

Cancer Cell Int

MiR-384 inhibits the proliferation of colorectal cancer by targeting AKT3.

Authors - Yong-Xia Wang