Name: ALDH2 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 15310-1-AP

Tested Applications

Applications:

WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee

ALDH2 Antibody WB mouse testis tissue 15310-1-AP

mouse testis tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP( ALDH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed A549 cells using 15310-1-AP(ALDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ALDH2 Antibody IP mouse lung tissue 15310-1-AP

IP Result of anti-ALDH2 (IP:15310-1-AP, 4ug; Detection:15310-1-AP 1:1000) with mouse lung tissue lysate 2800ug.

ALDH2 Antibody IHC human liver tissue 15310-1-AP

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP( ALDH2 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP( ALDH2 Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:

human, mouse, rat

Positive WB detected in:

mouse testis tissue, human heart tissue, human liver tissue, mouse lung tissue, mouse skeletal muscle tissue, rat lung tissue, HEK-293 cells, K-562 cells

Positive IP detected in:

mouse lung tissue

Positive IHC detected in:

human liver tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

A549 cells

Recommended dilution:

WB : 1:1000-1:4000

IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

IHC : 1:50-1:500

IF : 1:50-1:500

Sample-dependent, check data in Validation tab

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ALDH2 fusion protein Ag7452

Full name:

aldehyde dehydrogenase 2 family (mitochondrial)

Calculated molecular weight:

56 kDa

Observed molecular weight:

50-55 kDa

GenBank accession number:

BC002967

Gene ID (NCBI):

217

Gene symbol

ALDH2

Synonyms

ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM

Background

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Showing 1- [6] of [12] Publications

Author:		Sajin Bae
Pubmed ID:		28228507
Journal:		J Nutr
Title:		Alcohol Dehydrogenase 5 Is a Source of Formate for De Novo Purine Biosynthesis in HepG2 Cells.
Application:		WB
KD validated
Species:		human

Author:		Yusuke Amanuma
Pubmed ID:		26374466
Journal:		Sci Rep
Title:		Protective role of ALDH2 against acetaldehyde-derived DNA damage in oesophageal squamous epithelium.
Application:		WB
KO validated
Species:		human

Author:		Shanshan Zhong
Pubmed ID:		30375985
Journal:		J Clin Invest
Title:		Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.
Application:		WB,IP,IF
KO validated
Species:		human,mouse

Author:		Fei Wang
Pubmed ID:		29383448
Journal:		Mol Genet Genomics
Title:		A comparison of CRISPR/Cas9 and siRNA-mediated ALDH2 gene silencing in human cell lines.
Application:		WB
KO validated
Species:		human

Author:		Meng Li
Pubmed ID:		30671488
Journal:		J Immunol Res
Title:		Alda-1 Ameliorates Liver Ischemia-Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice.
Application:

Species:		mouse

Author:		Colin T Shearn
Pubmed ID:		27124661
Journal:		PLoS One
Title:		Liver-Specific Deletion of Phosphatase and Tensin Homolog Deleted on Chromosome 10 Significantly Ameliorates Chronic EtOH-Induced Increases in Hepatocellular Damage.
Application:		WB

Species:		mouse

WB Figures

ALDH2 Antibody WB HEK-293 cells 15310-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 15310-1-AP( ALDH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ALDH2 Antibody WB human heart tissue 15310-1-AP

human heart tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP(ALDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ALDH2 Antibody WB human liver tissue 15310-1-AP

human liver tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP(ALDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ALDH2 Antibody WB K-562 cells 15310-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 15310-1-AP( ALDH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ALDH2 Antibody WB mouse lung tissue 15310-1-AP

mouse lung tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP(ALDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ALDH2 Antibody WB mouse skeletal muscle tissue 15310-1-AP

mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP(ALDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse testis tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP( ALDH2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ALDH2 Antibody WB rat lung tissue 15310-1-AP

rat lung tissue were subjected to SDS PAGE followed by western blot with 15310-1-AP( ALDH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

ALDH2 Antibody IHC human heart tissue 15310-1-AP

Immunohistochemical analysis of paraffin-embedded human heart using 15310-1-AP(ALDH2 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human heart using 15310-1-AP(ALDH2 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical analysis of paraffin-embedded human liver using 15310-1-AP(ALDH2 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical analysis of paraffin-embedded human liver using 15310-1-AP(ALDH2 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP( ALDH2 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15310-1-AP( ALDH2 Antibody) at dilution of 1:200 (under 40x lens).

IP Figures

IP Result of anti-ALDH2 (IP:15310-1-AP, 4ug; Detection:15310-1-AP 1:1000) with mouse lung tissue lysate 2800ug.

IF Figures

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed A549 cells using 15310-1-AP(ALDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ALDH2 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 15310-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [12] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

Product-specific protocols

Standard Protocols

ALDH2 Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 15310-1-AP

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Special offer: Add control/tag antibody for $99

Tested Applications

Product Information

Immunogen Information

Background