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- KD/KO Validated
ALDH2 Polyclonal antibody
ALDH2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15310-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, human heart tissue, human liver tissue, mouse lung tissue, mouse skeletal muscle tissue, rat lung tissue, HEK-293 cells, K-562 cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human liver tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
15310-1-AP targets ALDH2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ALDH2 fusion protein Ag7452 |
| Full Name | aldehyde dehydrogenase 2 family (mitochondrial) |
| Calculated molecular weight | 56 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC002967 |
| Gene symbol | ALDH2 |
| Gene ID (NCBI) | 217 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| IHC protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| IF protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| IP protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunol Res Alda-1 Ameliorates Liver Ischemia-Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice. | ||
Mol Genet Genomics A comparison of CRISPR/Cas9 and siRNA-mediated ALDH2 gene silencing in human cell lines.
| ||
Mol Med Rep Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy. | ||
Int J Radiat Biol Combination of podophyllotoxin and rutin modulate radiation-induced alterations of jejunal proteome in mice. | ||
Am J Physiol Regul Integr Comp Physiol Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.
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