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  • KD/KO Validated

ALDH2 Polyclonal antibody

ALDH2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15310-1-AP

Synonyms

ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM



Tested Applications

Positive WB detected inmouse testis tissue, human heart tissue, human liver tissue, mouse lung tissue, mouse skeletal muscle tissue, rat lung tissue, HEK-293 cells, K-562 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman liver tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15310-1-AP targets ALDH2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH2 fusion protein Ag7452
Full Name aldehyde dehydrogenase 2 family (mitochondrial)
Calculated molecular weight 56 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC002967
Gene symbol ALDH2
Gene ID (NCBI) 217
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.

Protocols

Product Specific Protocols
WB protocol for ALDH2 antibody 15310-1-APDownload protocol
IHC protocol for ALDH2 antibody 15310-1-APDownload protocol
IF protocol for ALDH2 antibody 15310-1-APDownload protocol
IP protocol for ALDH2 antibody 15310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

J Immunol Res

Alda-1 Ameliorates Liver Ischemia-Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice.

Authors - Meng Li
humanWB

BMC Res Notes

Limitations of lymphoblastoid cell lines for functional analysis of SNPs.

Authors - Hansi Pathak
humanWB

Mol Genet Genomics

A comparison of CRISPR/Cas9 and siRNA-mediated ALDH2 gene silencing in human cell lines.

Authors - Fei Wang
  • KO Validated
ratWB

Mol Med Rep

Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy.

Authors - Zhongzhong Liu
mouseWB

Int J Radiat Biol

Combination of podophyllotoxin and rutin modulate radiation-induced alterations of jejunal proteome in mice.

Authors - Sania Bajaj
mouseWB

Am J Physiol Regul Integr Comp Physiol

Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.

Authors - Yuka Wakabayashi
  • KO Validated