Tested Applications
| Positive WB detected in | K-562 cells, HEK-293 cells, HepG2 cells, HuH-7 cells, mouse kidney tissue, mouse liver tisue, mouse testis tissue, rat kidney tissue, rat liver tissue, rat testis tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 14 publications below |
| WB | See 56 publications below |
| IHC | See 6 publications below |
| IF | See 8 publications below |
| IP | See 4 publications below |
| CoIP | See 2 publications below |
Product Information
15310-1-AP targets ALDH2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ALDH2 fusion protein Ag7452 Predict reactive species |
| Full Name | aldehyde dehydrogenase 2 family (mitochondrial) |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC002967 |
| Gene Symbol | ALDH2 |
| Gene ID (NCBI) | 217 |
| RRID | AB_2224185 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05091 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| IHC protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| IF protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| IP protocol for ALDH2 antibody 15310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Discov Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.
| ||
Blood Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.
| ||
Mol Cell Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.
| ||
Adv Sci (Weinh) Sirtuin 5-Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen-Induced Acute Liver Injury |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hua (Verified Customer) (03-26-2021) | Excellent antibody.
 |
