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ALDOA Polyclonal antibody

ALDOA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, Co-IP, ELISA

Conjugate

Unconjugated

Cat no : 11217-1-AP

Synonyms

ALDOA, Aldolase A



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, HepG2 cells, HeLa cells, mouse uterus tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11217-1-AP targets ALDOA in WB, IP, IHC, IF, FC, Co-IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDOA fusion protein Ag1726
Full Name aldolase A, fructose-bisphosphate
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC016800
Gene symbol ALDOA
Gene ID (NCBI) 226
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

Protocols

Product Specific Protocols
WB protocol for ALDOA antibody 11217-1-APDownload protocol
IHC protocol for ALDOA antibody 11217-1-APDownload protocol
IF protocol for ALDOA antibody 11217-1-APDownload protocol
IP protocol for ALDOA antibody 11217-1-APDownload protocol
FC protocol for ALDOA antibody 11217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Lab Invest

Accumulation of fructose 1,6-bisphosphate protects clear cell renal cell carcinoma from oxidative stress.

Authors - Jun Wang
mouseWB

Acta Biochim Biophys Sin (Shanghai)

Alteration of sperm protein profile induced by cigarette smoking.

Authors - Xiaohui Chen
WB,IF

Theriogenology

Characterization of GALNTL5 gene sequence and expression in ovine testes and sperm.

Authors - Xiaolei Yao
humanWB

Mol Cell Biochem

Exosomes carrying ALDOA and ALDH3A1 from irradiated lung cancer cells enhance migration and invasion of recipients by accelerating glycolysis.

Authors - Chen Wang
  • KD Validated
mouseWB

J Agric Food Chem

A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.

Authors - Cheng Liu
humanWB

Onco Targets Ther

Recombinant Viral Capsid Protein L2 (rVL2) of HPV 16 Suppresses Cell Proliferation and Glucose Metabolism via ITGB7/C/EBPβ Signaling Pathway in Cervical Cancer Cell Lines.

Authors - Zhihong Chai