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ALDOA Polyclonal antibody
ALDOA Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 11217-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, Jurkat cells, HepG2 cells, HeLa cells, mouse uterus tissue, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human colon cancer tissue, human lung cancer tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11217-1-AP targets ALDOA in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ALDOA fusion protein Ag1726|
|Full Name||aldolase A, fructose-bisphosphate|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||39 kDa|
|GenBank accession number||BC016800|
|Gene ID (NCBI)||226|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).
|Product Specific Protocols|
|WB protocol for ALDOA antibody 11217-1-AP||Download protocol|
|IHC protocol for ALDOA antibody 11217-1-AP||Download protocol|
|IF protocol for ALDOA antibody 11217-1-AP||Download protocol|
|IP protocol for ALDOA antibody 11217-1-AP||Download protocol|
|FC protocol for ALDOA antibody 11217-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Accumulation of fructose 1,6-bisphosphate protects clear cell renal cell carcinoma from oxidative stress.
Acta Biochim Biophys Sin (Shanghai)
Alteration of sperm protein profile induced by cigarette smoking.
Characterization of GALNTL5 gene sequence and expression in ovine testes and sperm.
Mol Cell Biochem
Exosomes carrying ALDOA and ALDH3A1 from irradiated lung cancer cells enhance migration and invasion of recipients by accelerating glycolysis.
J Agric Food Chem
A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.
Onco Targets Ther
Recombinant Viral Capsid Protein L2 (rVL2) of HPV 16 Suppresses Cell Proliferation and Glucose Metabolism via ITGB7/C/EBPβ Signaling Pathway in Cervical Cancer Cell Lines.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
K (Verified Customer) (12-20-2020)
Worked well for IHC at 1:200 dilution