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ALDOB Polyclonal antibody

ALDOB Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18065-1-AP

Synonyms

ALDB, ALDOB, Liver type aldolase



Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue, mouse brain tissue, Raji cells, mouse skeletal muscle tissue, rat kidney tissue
Positive IP detected inmouse brain tissue, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18065-1-AP targets ALDOB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDOB fusion protein Ag12695
Full Name aldolase B, fructose-bisphosphate
Calculated molecular weight 316 aa, 35 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC029399
Gene symbol ALDOB
Gene ID (NCBI) 229
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A , B(ALDOB), and C. Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183).

Protocols

Product Specific Protocols
WB protocol for ALDOB antibody 18065-1-APDownload protocol
IHC protocol for ALDOB antibody 18065-1-APDownload protocol
IF protocol for ALDOB antibody 18065-1-APDownload protocol
IP protocol for ALDOB antibody 18065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Free Radic Biol Med

SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.

Authors - Yuanzhen Ma
humanWB,IF

Lab Invest

Accumulation of fructose 1,6-bisphosphate protects clear cell renal cell carcinoma from oxidative stress.

Authors - Jun Wang

J Clin Endocrinol Metab

PMID: 30202879

Authors - Felicia Gerst
WB

Sci Rep

Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus.

Authors - Chang Zhao
humanWB, IHC

Mol Cancer

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.

Authors - Qi-Fei Tao
ratWB

Oncotarget

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Authors - Hao-Long Zeng