Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse liver tissue, mouse brain tissue, Raji cells, mouse skeletal muscle tissue, rat kidney tissue |
| Positive IP detected in | mouse brain tissue, mouse kidney tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18065-1-AP targets ALDOB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12695 Product name: Recombinant human ALDOB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC029399 Sequence: MAHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLPIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSGVARLQTRRQPRRLL Predict reactive species |
| Full Name | aldolase B, fructose-bisphosphate |
| Calculated Molecular Weight | 316 aa, 35 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC029399 |
| Gene Symbol | ALDOB |
| Gene ID (NCBI) | 229 |
| RRID | AB_2273968 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05062 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ALDOB antibody 18065-1-AP | Download protocol |
| IP protocol for ALDOB antibody 18065-1-AP | Download protocol |
| WB protocol for ALDOB antibody 18065-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Aldolase B (ALDOB) antibody (Cat# 18065-1-AP) has accumulated 46 citations published in high-impact journals including Nature, Signal Transduction and Targeted Therapy, Cell Metabolism, Nature Communications, Cell Research, Nature Metabolism, Molecular Cancer, PLoS Biology, and EMBO Reports. Associated research fields encompass oncology (hepatocellular, colorectal, renal, pancreatic, gastric, and lung cancers), metabolic biology (glycolysis, fructose metabolism, AMPK signaling), endocrinology (insulin secretion, diabetes), liver disease (steatohepatitis), nephrology, and neurology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Fructose 1-phosphate inhibits mannose phosphate isomerase to suppress hepatocellular carcinogenesis | ||
Signal Transduct Target Ther Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes | ||
Cancer Cell Proteogenomic characterization identifies clinically relevant subgroups of intrahepatic cholangiocarcinoma. | ||
Nature Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.
| ||
Mol Cancer Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma. | ||
Cell Metab Fructose-1,6-bisphosphatase is a nonenzymatic safety valve that curtails AKT activation to prevent insulin hyperresponsiveness |
