|Positive WB detected in||mouse kidney tissue, mouse liver tissue, mouse brain tissue, Raji cells, mouse skeletal muscle tissue, rat kidney tissue|
|Positive IP detected in||mouse brain tissue, mouse kidney tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18065-1-AP targets ALDOB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ALDOB fusion protein Ag12695|
|Full Name||aldolase B, fructose-bisphosphate|
|Calculated molecular weight||316 aa, 35 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||BC029399|
|Gene ID (NCBI)||229|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A , B(ALDOB), and C. Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183).
|Product Specific Protocols|
|WB protocol for ALDOB antibody 18065-1-AP||Download protocol|
|IHC protocol for ALDOB antibody 18065-1-AP||Download protocol|
|IF protocol for ALDOB antibody 18065-1-AP||Download protocol|
|IP protocol for ALDOB antibody 18065-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Free Radic Biol Med
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Accumulation of fructose 1,6-bisphosphate protects clear cell renal cell carcinoma from oxidative stress.
J Clin Endocrinol Metab
Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus.
Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.
Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.