Tested Applications
| Positive WB detected in | HeLa cells, human placenta tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15092-1-AP targets ALG2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4833 Product name: Recombinant human ALG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 32-323 aa of BC015126 Sequence: RLARRRKKILFYCHFPDLLLTKRDSFLKRLYRAPIDWIEEYTTGMADCILVNSQFTAAVFKETFKSLSHIDPDVLYPSLNVTSFDSVVPEKLDDLVPKGKKFLLLSINRYERKKNLTLALEALVQLRGRLTSQDWERVHLIVAGGYDERVLENVEHYQELKKMVQQSDLGQYVTFLRSFSDKQKISLLHSCTCVLYTPSNEHFGIVPLEAMYMQCPVIAVNSGGPLESIDHSVTGFLCEPDPVHFSEAIEKFIREPSLKATMGLAGRARVKEKFSPEAFTEQLYRYVTKLLV Predict reactive species |
| Full Name | asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae) |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 47 kDa, 37 kDa |
| GenBank Accession Number | BC015126 |
| Gene Symbol | ALG2 |
| Gene ID (NCBI) | 85365 |
| RRID | AB_2878108 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H553 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ALG2 antibody 15092-1-AP | Download protocol |
| WB protocol for ALG2 antibody 15092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALG2 polyclonal antibody (Cat# 15092-1-AP) has 2 citations. They appear in EMBO Journal and bioRxiv, both by Jacob Duran (2024). The research focuses on lysosomal damage signaling, specifically how calcium release from damaged lysosomes triggers cytoprotective stress granule formation for cell survival. The antibody was used in Western blot applications with human samples.
| Species | Application | Title |
|---|---|---|
bioRxiv A mechanism that transduces lysosomal damage signals to stress granule formation for cell survival |
