ALK/CD246 Monoclonal antibody

ALK/CD246 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

7G9E3

Cat no : 60321-1-Ig

Synonyms

ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, TFG/ALK



Tested Applications

Positive WB detected inRecombinant protein
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60321-1-Ig targets ALK/CD246 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ALK/CD246 fusion protein Ag21493
Full Name anaplastic lymphoma receptor tyrosine kinase
Calculated molecular weight 1620 aa, 176 kDa
GenBank accession numberNM_004304
Gene symbol ALK
Gene ID (NCBI) 238
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The EML4 (echinoderm microtubule-associated protein-like 4)-ALK fusion protein have been described in non-small-cell lung cancer (NSCLC), this transforming fusion kinase is a promising candidate for a therapeutic target as well as for a diagnostic molecular marker in NSCLC (PMID: 17625570).

Protocols

Product Specific Protocols
WB protocol for ALK/CD246 antibody 60321-1-IgDownload protocol
IHC protocol for ALK/CD246 antibody 60321-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols