Tested Applications
| Positive WB detected in | DU 145 cells, HL-60 cells, K-562 cells, PC-3 cells, human placenta tissue |
| Positive IHC detected in | human lung cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10021-1-Ig targets 5 Lipoxygenase in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | arachidonate 5-lipoxygenase |
| Calculated Molecular Weight | 674 aa, 78 kDa |
| Observed Molecular Weight | 70-78 kDa |
| GenBank Accession Number | BC130332 |
| Gene Symbol | 5 Lipoxygenase |
| Gene ID (NCBI) | 240 |
| RRID | AB_513237 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09917 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for 5 Lipoxygenase antibody 10021-1-Ig | Download protocol |
| IHC protocol for 5 Lipoxygenase antibody 10021-1-Ig | Download protocol |
| WB protocol for 5 Lipoxygenase antibody 10021-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ALOX5 (5-Lipoxygenase) antibody (Cat# 10021-1-Ig) has 31 citations published in journals including Nature Communications, Journal of Biological Chemistry, Journal of Translational Medicine, International Immunopharmacology, Frontiers in Pharmacology, Molecular Neurobiology, and Scientific Reports. Research fields span oncology (prostate, ovarian, pancreatic, renal, liver cancers), ferroptosis, lipid metabolism, neuroinflammation, immune regulation, stroke, atherosclerosis, and liver disease.
| Species | Application | Title |
|---|---|---|
Nat Commun Inhibition of neutrophil swarming by type I interferon promotes intracellular bacterial evasion | ||
Nat Commun AI-guided CRISPR screening reveals therapeutic targets in psoriasis.
| ||
Cell Commun Signal Whole-gene CRISPR/cas9 library screen revealed targeting STAT6 increased the sensitivity of liver cancer to celecoxib via inhibiting arachidonic acid shunting. | ||
J Neuroinflammation P2X7-CaMKII drives 5-LOX nuclear translocation to impair microglial function after subarachnoid hemorrhage. | ||
NPJ Sci Food Integratomic investigation to assess the liver lipidomic and antiinflammatory activity of lupin protein hydrolysate. |
