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AMIGO1 Polyclonal antibody

AMIGO1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 21874-1-AP

Synonyms

ALI2, Alivin 2, AMIGO, AMIGO 1, AMIGO1, Amphoterin induced protein 1, KIAA1163

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using AMIGO1 Polyclonal antibody (21874-1-AP)

    WB analysis of mouse brain using 21874-1-AP

    mouse brain tissue and rat brain tissue were subjected to SDS PAGE followed by western blot with 21874-1-AP (AMIGO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21874-1-AP is AMIGO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMIGO1 fusion protein Ag16226
Full Name adhesion molecule with Ig-like domain 1
Calculated Molecular Weight 493 aa, 55 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC040879
Gene Symbol AMIGO1
Gene ID (NCBI) 57463
RRIDAB_2878935
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMIGO1 is a brain-enriched transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRR) and a single immunoglobulin-like (Ig) domain (PMID: 21938721). It is widely expressed in the central nervous system (CNS), and can be found in neurons, astrocytes as well as oligodendrocytes. AMIGO1 acts as a homophilic adhesion molecule that induces outgrowth and fasciculation of neurites in central neurons (PMID: 12629050). It has been reported that AMIGO1 is a glycosylated protein, the apparent molecular weight (70-80 kDa) is much higher than the predicted molecular size of AMIGO1 (55 kDa) (PMID: 12629050; 21938721; 22056818).

Protocols

Product Specific Protocols
WB protocol for AMIGO1 antibody 21874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols