Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21874-1-AP targets AMIGO1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16226 Product name: Recombinant human AMIGO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 224-493 aa of BC040879 Sequence: NCDCELYQLFSHWQYRQLSSVMDFQEDLYCMNSKKLHNVFNLSFLNCGEYKERAWEAHLGDTLIIKCDTKQQGMTKVWVTPSNERVLDEVTNGTVSVSKDGSLLFQQVQVEDGGVYTCYAMGETFNETLSVELKVHNFTLHGHHDTLNTAYTTLVGCILSVVLVLIYLYLTPCRCWCRGVEKPSSHQGDSLSSSMLSTTPNHDPMAGGDKDDGFDRRVAFLEPAGPGQGQSGKLKPGNTLPVPEATGKGQRRMSDPESVSSVFSDTPIVV Predict reactive species |
| Full Name | adhesion molecule with Ig-like domain 1 |
| Calculated Molecular Weight | 493 aa, 55 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC040879 |
| Gene Symbol | AMIGO1 |
| Gene ID (NCBI) | 57463 |
| RRID | AB_2878935 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86WK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AMIGO1 is a brain-enriched transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRR) and a single immunoglobulin-like (Ig) domain (PMID: 21938721). It is widely expressed in the central nervous system (CNS), and can be found in neurons, astrocytes as well as oligodendrocytes. AMIGO1 acts as a homophilic adhesion molecule that induces outgrowth and fasciculation of neurites in central neurons (PMID: 12629050). It has been reported that AMIGO1 is a glycosylated protein, the apparent molecular weight (70-80 kDa) is much higher than the predicted molecular size of AMIGO1 (55 kDa) (PMID: 12629050; 21938721; 22056818).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AMIGO1 antibody 21874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
