Angiopoietin 2 Polyclonal antibody

Angiopoietin 2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24613-1-AP

Synonyms

AGPT2, ANG 2, ANG2, angiopoietin 2, ANGPT2



Tested Applications

Positive WB detected inHUVEC cells, HeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24613-1-AP targets Angiopoietin 2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Angiopoietin 2 fusion protein Ag17938
Full Name angiopoietin 2
Calculated Molecular Weight 496 aa, 57 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC126200
Gene Symbol ANGPT2
Gene ID (NCBI) 285
RRIDAB_2879639
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin-2 (Ang2) is a growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, one of the main pathways involved in angiogenesis. Angiopoietin-2 was identified through a cDNA library screening, shortly after the identification of angiopoietin-1. Angiopoietin-2, is a natural antagonist for Tie2 that disrupts in vivo angiogenesis. In adult mice and humans, Angiopoietin-2 is expressed only at sites of vascular remodeling. Angiopoietin-2 has also been found to be highly expressed in diverse tumor cells and plays an important role in tumor angiogenesis and inflammation.

Protocols

Product Specific Protocols
WB protocol for Angiopoietin 2 antibody 24613-1-APDownload protocol
IHC protocol for Angiopoietin 2 antibody 24613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Ther Nucleic Acids

Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.

Authors - Ying Wang
humanWB

J Med Chem

Discovery of Novel Small-Molecule Antiangiogenesis Agents to Treat Diabetic Retinopathy.

Authors - Donghwa Kim
humanWB

Int J Mol Sci

MiR-29a Curbs Hepatocellular Carcinoma Incidence via Targeting of HIF-1α and ANGPT2.

Authors - Ying-Hsien Huang
mouseWB,IF

Exp Mol Med

Akt1-dependent expression of angiopoietin 1 and 2 in vascular smooth muscle cells leads to vascular stabilization

Authors - Jung Min Ha
humanIHC

Front Oncol

MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis.

Authors - Hailin Li
humanIF

Biomed Pharmacother

Diosmetin inhibits tumor development and block tumor angiogenesis in skin cancer.

Authors - Jawun Choi