Tested Applications
| Positive WB detected in | HUVEC cells, HeLa cells, HepG2 cells, K-562 cells |
| Positive IF/ICC detected in | PC-12 cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 22 publications below |
| IHC | See 8 publications below |
| IF | See 7 publications below |
Product Information
24613-1-AP targets Angiopoietin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17938 Product name: Recombinant human ANGPT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 357-443 aa of BC126200 Sequence: NEFVSQLTNQQRYVLKIHLKDWEGNEAYSLYEHFYLSSEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDGDNDKCICKCSQMLTG Predict reactive species |
| Full Name | angiopoietin 2 |
| Calculated Molecular Weight | 496 aa, 57 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC126200 |
| Gene Symbol | Angiopoietin 2 |
| Gene ID (NCBI) | 285 |
| RRID | AB_2879639 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15123 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Angiopoietin-2 (Ang2) is a growth factor belonging to the angiopoietin/Tie (tyrosine kinase with Ig and EGF homology domains) signaling pathway, one of the main pathways involved in angiogenesis. Angiopoietin-2 was identified through a cDNA library screening, shortly after the identification of angiopoietin-1. Angiopoietin-2, is a natural antagonist for Tie2 that disrupts in vivo angiogenesis. In adult mice and humans, Angiopoietin-2 is expressed only at sites of vascular remodeling. Angiopoietin-2 has also been found to be highly expressed in diverse tumor cells and plays an important role in tumor angiogenesis and inflammation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Angiopoietin 2 antibody 24613-1-AP | Download protocol |
| WB protocol for Angiopoietin 2 antibody 24613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC. | ||
J Med Chem Discovery of Novel Small-Molecule Antiangiogenesis Agents to Treat Diabetic Retinopathy. | ||
Int J Mol Sci MiR-29a Curbs Hepatocellular Carcinoma Incidence via Targeting of HIF-1α and ANGPT2. | ||
Exp Mol Med Akt1-dependent expression of angiopoietin 1 and 2 in vascular smooth muscle cells leads to vascular stabilization | ||
Front Oncol MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis. | ||
Biomed Pharmacother Diosmetin inhibits tumor development and block tumor angiogenesis in skin cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Angie (Verified Customer) (07-25-2025) | The antibody detected several bands at dilution of 1:2000 incubated at room temperature for 2 hours followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 1 hour at room temperature.
 |
FH Marine (Verified Customer) (07-01-2025) | It is not specific, it binds at different molecular weight on the ladder
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