ANKRD1 Polyclonal antibody
ANKRD1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 11427-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IF | See 3 publications below |
Product Information
11427-1-AP targets ANKRD1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ANKRD1 fusion protein Ag1970 |
| Full Name | ankyrin repeat domain 1 (cardiac muscle) |
| Calculated Molecular Weight | 319 aa, 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC018667 |
| Gene Symbol | ANKRD1 |
| Gene ID (NCBI) | 27063 |
| RRID | AB_2227402 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression. This is a rabbit polyclonal antibody produced by the full-length ANKRD1 of human origin
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANKRD1 antibody 11427-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer. | ||
Hum Mol Genet Exploiting The CRISPR/CAS9 System to Study Alternative Splicing In Vivo: Application to Titin. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Luigi (Verified Customer) (11-29-2018) |
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