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ANKS1B Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 24783-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

ANKS1B Antibody WB A549 cells 24783-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 24783-1-AP( ANKS1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ANKS1B Antibody IP HeLa cells 24783-1-AP

IP Result of anti-ANKS1B (IP:24783-1-AP, 4ug; Detection:24783-1-AP 1:1000) with HeLa cells lysate 3200ug.

ANKS1B Antibody IHC human testis tissue 24783-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 24783-1-AP( ANKS1B Antibody) at dilution of 1:50 (under 10x lens)

ANKS1B Antibody IHC human testis tissue 24783-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 24783-1-AP( ANKS1B Antibody) at dilution of 1:50 (under 40x lens)

ANKS1B Antibody IF HepG2 cells 24783-1-AP

Immunofluorescent analysis of HepG2 cells using 24783-1-AP(ANKS1B antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, mouse
Positive WB detected in:
A549 cells, HeLa cells, mouse testis tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ANKS1B fusion protein Ag20454
Full name:
ankyrin repeat and sterile alpha motif domain containing 1B
Calculated molecular weight:
1248aa,138 kDa
Observed molecular weight:
65-70 kDa
GenBank accession number:
BC026313
Gene ID (NCBI):
56899
Gene symbol
ANKS1B
Synonyms
AIDA, AIDA 1, ANKS1B, ANKS2, cajalin 2, E2A PBX1 associated protein, EB 1, EB1

Background

ANKS1B also named as AIDA or cajalin 2 is a amino acid protein, which contains 7 ANK repeats, 1 PID domain and 2 SAM domians. ANKS1B localizes in cytoplasm and is highly expressed in brain and testis. ANKS1B exists as many alternatively spliced isoforms. It interacts with AbPP to play a role in brain development. AIDA-1 also interacts with coilin in Cajal bodies to regulate pre-mRNA splicing.


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