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- KD/KO Validated
ANP32B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10843-1-AP
ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29
Validation Data Gallery
|Positive WB detected in||HEK-293T cells, RAW 264.7 cells, HEK-293 cells, A431 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10843-1-AP targets ANP32B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ANP32B fusion protein Ag1274|
|Full Name||acidic (leucine-rich) nuclear phosphoprotein 32 family, member B|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||29 kDa|
|GenBank accession number||BC013003|
|Gene ID (NCBI)||10541|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).
|Product Specific Protocols|
|WB protocol for ANP32B antibody 10843-1-AP||Download protocol|
|IHC protocol for ANP32B antibody 10843-1-AP||Download protocol|
|IF protocol for ANP32B antibody 10843-1-AP||Download protocol|
|IP protocol for ANP32B antibody 10843-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Med Rep
Caspase-3‑resistant uncleavable form of acidic leucine-rich nuclear phosphoprotein 32B potentiates leukemic cell apoptosis.
Biochem Biophys Res Commun
Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) contributes to retinoic acid-induced differentiation of leukemic cells.
Generation and characterization of the Anp32e-deficient mouse.
ANP32 proteins are essential for influenza virus replication in human cells.
Cell Death Dis
ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.
Blood Cancer J