AP3B2 Antibody

AP3B2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 18331-1-AP

Synonyms

AP 3 complex subunit beta 2, AP3B2, Beta 3B adaptin, NAPTB



Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The 18331-1-AP immunogen is Fusion Protein AP3B2 protein expressed in E. coli.

Reactivity human, mouse, rat
Cited Species human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP3B2 fusion protein Ag12861
Full Name adaptor-related protein complex 3, beta 2 subunit
Calculated molecular weight 1082aa,119 kDa
Observed molecular weight 120-140 kDa
GenBank accession number BC093739
Gene symbol AP3B2
Gene ID (NCBI) 8120
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP3B2 is a subunit of the AP-3 complex which is composed of two large adaptins (AP3D1 and AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP-3 complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Protocols

Product Specific Protocols
WB protocol for AP3B2 antibody 18331-1-APDownload protocol
IP protocol for AP3B2 antibody 18331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Neurology

Autoimmune gait disturbance accompanying adaptor protein-3B2-IgG.

Authors - Josephe A Honorat