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AP4M1 Polyclonal antibody

AP4M1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11653-1-AP

Synonyms

AP 4 complex subunit mu 1, AP4M1, MU 4, Mu adaptin related protein 2, MU ARP2, Mu subunit of AP 4, mu4, Mu4 adaptin, MUARP2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using AP4M1 Polyclonal antibody (11653-1-AP)

    WB analysis of mouse heart using 11653-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11653-1-AP (AP4M1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using AP4M1 Polyclonal antibody (11653-1-AP)

    WB analysis of A431 using 11653-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11653-1-AP (AP4M1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AP4M1 Polyclonal antibody (11653-1-AP)

    WB analysis of HeLa using 11653-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11653-1-AP (AP4M1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using AP4M1 Polyclonal antibody (11653-1-AP)

    WB analysis of mouse lung using 11653-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11653-1-AP (AP4M1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using AP4M1 Polyclonal antibody (11653-1-AP)

    IHC staining of human lymphoma using 11653-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11653-1-AP (AP4M1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using AP4M1 Polyclonal antibody (11653-1-AP)

    IHC staining of human lymphoma using 11653-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11653-1-AP (AP4M1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, mouse lung tissue, A431 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11653-1-AP targets AP4M1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP4M1 fusion protein Ag2222
Full Name adaptor-related protein complex 4, mu 1 subunit
Calculated Molecular Weight 453 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC018705
Gene Symbol AP4M1
Gene ID (NCBI) 9179
RRIDAB_2227267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4M1 is a member of the AP4 complex, which also includes AP4B1, AP4E1, and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4M1.

Protocols

Product Specific Protocols
WB protocol for AP4M1 antibody 11653-1-APDownload protocol
IHC protocol for AP4M1 antibody 11653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell Int

AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma

Authors - Yuanhao Peng
View Article