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  • Western Blot (WB) analysis of human plasma using APOD Recombinant antibody (86500-1-RR)

    WB analysis of human plasma using 86500-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86500-1-RR (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using APOD Recombinant antibody (86500-1-RR)

    WB analysis of human placenta using 86500-1-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 86500-1-RR (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using APOD Recombinant antibody (86500-1-RR)

    WB analysis using 86500-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86500-1-RR (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86500-1-RR

    Affinity and Kinetic Characterization of 86500-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86500-1-RR against Human APOD were performed. The affinity constant is below 1 pM.

APOD Recombinant monoclonal antibody

APOD Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86500-1-RR
Clone No.251229A10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Apo D, Apo-D, apolipoprotein D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman plasma, human placenta tissue, human urine

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86500-1-RR targets APOD in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0812

Product name: Recombinant human APOD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 15-189 aa of BC007402

Sequence: FGAAEGQAFHLGKCPNPPVQENFDVNKYLGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYWILATDYENYALVYSCTCIIQLFHVDFAWILARNPNLPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLS

Predict reactive species
Full Name apolipoprotein D
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 21-33 kDa
GenBank Accession NumberBC007402
Gene Symbol APOD
Gene ID (NCBI) 347
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP05090
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.

Protocols

Product Specific Protocols
WB protocol for APOD antibody 86500-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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