ARC Monoclonal antibody

ARC Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1G4B5

Cat no : 66550-1-Ig

Synonyms

ARC, ARC/ARG3.1, Arg3.1, KIAA0278



Tested Applications

Positive WB detected inHEK-293 cells, pig brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66550-1-Ig targets ARC in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARC fusion protein Ag9445
Full Name activity-regulated cytoskeleton-associated protein
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC012321
Gene symbol ARC
Gene ID (NCBI) 23237
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.

Protocols

Product Specific Protocols
WB protocol for ARC antibody 66550-1-IgDownload protocol
IHC protocol for ARC antibody 66550-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols