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ARCN1 Polyclonal antibody

ARCN1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23843-1-AP

Synonyms

Archain, archain 1, ARCN1, Coatomer subunit delta, COPD, Delta coat protein, Delta COP

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ARCN1 Polyclonal antibody (23843-1-AP)

    WB analysis of HepG2 using 23843-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ARCN1 Polyclonal antibody (23843-1-AP)

    WB analysis using 23843-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ARCN1 Polyclonal antibody (23843-1-AP)

    WB analysis of A549 using 23843-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ARCN1 Polyclonal antibody (23843-1-AP)

    IHC staining of human testis using 23843-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23843-1-AP (ARCN1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ARCN1 Polyclonal antibody (23843-1-AP)

    IHC staining of human testis using 23843-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23843-1-AP (ARCN1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARCN1 Polyclonal antibody (23843-1-AP)

    IF Staining of HepG2 using 23843-1-AP

    Immunofluorescent analysis of HepG2 cells using 23843-1-AP (ARCN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, A549 cells, Jurkat cells, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23843-1-AP targets ARCN1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARCN1 fusion protein Ag20836
Full Name archain 1
Calculated Molecular Weight 511 aa, 57 kDa
Observed Molecular Weight 57 kDa, 47 kDa
GenBank Accession NumberBC093636
Gene Symbol ARCN1
Gene ID (NCBI) 372
RRIDAB_2879337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Archain 1 (ARCN1), also known as delta-COP, is a subunit of the coat protein I (COPI) complex, which comprises one of the protein coats that mediate vesicle budding from the membrane. Protein sequences of ARCN1 are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. The gene of ARCN1 has been identified in chromosome band 11q23.3, and sequencing of cDNAs suggests that at least two forms of mRNA with alternative 5' ends are present. (PMID: 7782067; 20502676)

Protocols

Product Specific Protocols
WB protocol for ARCN1 antibody 23843-1-APDownload protocol
IHC protocol for ARCN1 antibody 23843-1-APDownload protocol
IF protocol for ARCN1 antibody 23843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nature

Cell stress in cortical organoids impairs molecular subtype specification.

Authors - Aparna Bhaduri
View Article
humanWB

Nat Chem Biol

Targeting colorectal cancer with small-molecule inhibitors of ALDH1B1.

Authors - Zhiping Feng
View Article
humanIF

bioRxiv

Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes.

Authors - Madeline G Andrews
View Article
humanWB

Sci Adv

HPV is a cargo for the COPI sorting complex during virus entry

Authors - Mara C Harwood
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (05-14-2021)

very good for WB detection

  • Applications: Western Blot