ARCN1 Polyclonal antibody

ARCN1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23843-1-AP

Synonyms

Archain, archain 1, ARCN1, Coatomer subunit delta, COPD, Delta coat protein, Delta COP



Tested Applications

Positive WB detected inHepG2 cells, A549 cells, mouse pancreas tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23843-1-AP is ARCN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARCN1 fusion protein Ag20836
Full Name archain 1
Calculated molecular weight 511 aa, 57 kDa
Observed molecular weight 57 kDa, 47 kDa
GenBank accession numberBC093636
Gene symbol ARCN1
Gene ID (NCBI) 372
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Archain 1 (ARCN1), also known as delta-COP, is a subunit of the coat protein I (COPI) complex, which comprises one of the protein coats that mediate vesicle budding from the membrane. Protein sequences of ARCN1 are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. The gene of ARCN1 has been identified in chromosome band 11q23.3, and sequencing of cDNAs suggests that at least two forms of mRNA with alternative 5' ends are present. (PMID: 7782067; 20502676)

Protocols

Product Specific Protocols
WB protocol for ARCN1 antibody 23843-1-APDownload protocol
IHC protocol for ARCN1 antibody 23843-1-APDownload protocol
IF protocol for ARCN1 antibody 23843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols