|Positive WB detected in||HeLa cells, COS-7 cells, HEK-293 cells, mouse brain tissue, rat brain tissue|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10790-1-AP targets ARF1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARF1 fusion protein Ag1244|
|Full Name||ADP-ribosylation factor 1|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||18-21 kDa|
|GenBank accession number||BC009247|
|Gene ID (NCBI)||375|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment. (PMID: 7759471, PMID: 16042562).This antibody can bind ARFs for the close sequences.
ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer.
Cancer Biol Ther
The small GTPase ADP-Ribosylation Factor 1 mediates the sensitivity of triple negative breast cancer cells to EGFR tyrosine kinase inhibitors.
ARF GTPases control phenotypic switching of vascular smooth muscle cells through the regulation of actin function and actin dependent gene expression.
ARF1 controls Rac1 signaling to regulate migration of MDA-MB-231 invasive breast cancer cells.
Mol Biol Cell
ARF1 regulates the Rho/MLC pathway to control EGF-dependent breast cancer cell invasion.
J Biol Chem
The adaptor proteins p66Shc and Grb2 regulate the activation of the GTPases ARF1 and ARF6 in invasive breast cancer cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Cristian (Verified Customer) (04-26-2019)
Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)
Boyan (Verified Customer) (01-25-2019)
By IF, PFA fixation, this antibody gave a Actin-like staining, but in literature and also its data sheet, it should be localised to the Golgi.