ARHGAP29 Polyclonal antibody

ARHGAP29 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12583-1-AP

Synonyms

ARHGAP29, PARG1, RP11 255E17.1



Tested Applications

Positive WB detected inHCT 116 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12583-1-AP targets ARHGAP29 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP29 fusion protein Ag3282
Full Name Rho GTPase activating protein 29
Calculated Molecular Weight 142 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC022483
Gene Symbol ARHGAP29
Gene ID (NCBI) 9411
RRIDAB_2918030
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARHGAP29 antibody 12583-1-APDownload protocol
IHC protocol for ARHGAP29 antibody 12583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Identification of Radil as a Ras binding partner and putative activator.

Authors - Byeong Hyeok Choi
humanWB

Hepatol Res

circTMEM181 upregulates ARHGAP29 to inhibit hepatocellular carcinoma migration and invasion by sponging miR-519a-5p

Authors - Bing Chen