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ARHGAP4 Polyclonal antibody

ARHGAP4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16697-1-AP

Synonyms

ARHGAP4, C1, KIAA0131, p115, RGC1, RhoGAP4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGAP4 Polyclonal antibody (16697-1-AP)

    WB analysis using 16697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16697-1-AP (ARHGAP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using ARHGAP4 Polyclonal antibody (16697-1-AP)

    IP experiment of HL-60 using 16697-1-AP

    IP result of anti-ARHGAP4 (IP:16697-1-AP, 4ug; Detection:16697-1-AP 1:500) with HL-60 cells lysate 1000ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ARHGAP4 Polyclonal antibody (16697-1-AP)

    IHC staining of human lymphoma using 16697-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using ARHGAP4 Polyclonal antibody (16697-1-AP)

    IHC staining of human lymphoma using 16697-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using ARHGAP4 Polyclonal antibody (16697-1-AP)

    IHC staining of mouse ovary using 16697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using ARHGAP4 Polyclonal antibody (16697-1-AP)

    IHC staining of mouse ovary using 16697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16697-1-AP (ARHGAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ARHGAP4 Polyclonal antibody (16697-1-AP)

    IF Staining of Jurkat using 16697-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP4 antibody (16697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells, K-562 cells, human placenta tissue, mouse spleen tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman lymphoma tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16697-1-AP targets ARHGAP4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP4 fusion protein Ag10091
Full Name Rho GTPase activating protein 4
Calculated Molecular Weight 986 aa, 109 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC052303
Gene Symbol ARHGAP4
Gene ID (NCBI) 393
RRIDAB_1850892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IHC protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IF protocol for ARHGAP4 antibody 16697-1-APDownload protocol
IP protocol for ARHGAP4 antibody 16697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Oncol

Analysis of ARHGAP4 Expression With Colorectal Cancer Clinical Characteristics and Prognosis.

Authors - Ming-Sheng Fu
View Article
humanWB,IHC

Life Sci

Prognosis, immune microenvironment, and personalized treatment prediction in Rho GTPase-activating protein 4-mutant cervical cancer: Computer strategies for precision oncology

Authors - Xiaoqin Lu
View Article