Tested Applications
| Positive WB detected in | HL-60 cells, Jurkat cells, K-562 cells, human placenta tissue, mouse spleen tissue |
| Positive IP detected in | HL-60 cells |
| Positive IHC detected in | human lymphoma tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16697-1-AP targets ARHGAP4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10091 Product name: Recombinant human ARHGAP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 714-986 aa of BC052303 Sequence: VALQGRVNQLVQTLIVQPDRVFPPLTSLPGPVYEKCMAPPSASCLGDAQLESLGADNEPELEAEMPAQEDDLEGVVEAVACFAYTGRTAQELSFRRGDVLRLHERASSDWWRGEHNGMRGLIPHKYITLPAGTEKQVVGAGLQTAGESGSSPEGLLASELVHRPEPCTSPEAMGPSGHRRRCLVPASPEQHVEVDKAVAQNMDSVFKELLGKTSVRQGLGPASTTSPSPGPRSPKAPPSSRLGRNKGFSRGPGAPASPSASHPQGLDTTPKPH Predict reactive species |
| Full Name | Rho GTPase activating protein 4 |
| Calculated Molecular Weight | 986 aa, 109 kDa |
| Observed Molecular Weight | 115 kDa |
| GenBank Accession Number | BC052303 |
| Gene Symbol | ARHGAP4 |
| Gene ID (NCBI) | 393 |
| RRID | AB_1850892 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P98171 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARHGAP4 antibody 16697-1-AP | Download protocol |
| IHC protocol for ARHGAP4 antibody 16697-1-AP | Download protocol |
| IP protocol for ARHGAP4 antibody 16697-1-AP | Download protocol |
| WB protocol for ARHGAP4 antibody 16697-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARHGAP4 Polyclonal antibody (Cat# 16697-1-AP) has 3 total citations. These appear in Life Sciences, Frontiers in Oncology, and Cancer Biomarkers. The associated research fields center on oncology, specifically examining ARHGAP4 expression and its prognostic significance in cervical cancer, colorectal cancer, and kidney renal clear cell carcinoma, with focus on immune microenvironment analysis and immune infiltration patterns.
| Species | Application | Title |
|---|---|---|
Life Sci Prognosis, immune microenvironment, and personalized treatment prediction in Rho GTPase-activating protein 4-mutant cervical cancer: Computer strategies for precision oncology | ||
Front Oncol Analysis of ARHGAP4 Expression With Colorectal Cancer Clinical Characteristics and Prognosis. | ||
Cancer Biomark Rho GTPase-activating protein 4 is upregulated in Kidney Renal Clear Cell Carcinoma and associated with poor prognosis and immune infiltration |
