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ARHGAP9 Polyclonal antibody

ARHGAP9 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 15665-1-AP

Synonyms

10C, ARHGAP9, RGL1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    WB analysis of Jurkat using 15665-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    WB analysis of HL-60 using 15665-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    WB analysis of HeLa using 15665-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15665-1-AP (ARHGAP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARHGAP9 Polyclonal antibody (15665-1-AP)

    IF Staining of HeLa using 15665-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15665-1-AP (ARHGAP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HL-60 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15665-1-AP targets ARHGAP9 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP9 fusion protein Ag8104
Full Name Rho GTPase activating protein 9
Calculated Molecular Weight 750 aa, 83 kDa
Observed Molecular Weight 70 kDa, 65 kDa
GenBank Accession NumberBC006107
Gene Symbol ARHGAP9
Gene ID (NCBI) 64333
RRIDAB_2059566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP9 belongs to RHO family of small GTPases, which inactivate RHO GTPases by stimulating their intrinsic GTPase activity. Several ARHGAP9 transcript isoforms were reported: including calculated mass of 81-83 kDa, 71 kDa and 61-63 kDa.

Protocols

Product Specific Protocols
WB protocol for ARHGAP9 antibody 15665-1-APDownload protocol
IF protocol for ARHGAP9 antibody 15665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genomics

ARHGAP9 knockdown promotes lung adenocarcinoma metastasis by activating Wnt/β-catenin signaling pathway via suppressing DKK2

Authors - Wenping Song
View Article