|Positive WB detected in||K-562 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14068-1-AP targets ARID3A in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARID3A fusion protein Ag5124|
|Full Name||AT rich interactive domain 3A (BRIGHT-like)|
|Calculated molecular weight||63 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC060828|
|Gene ID (NCBI)||1820|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) [PMID:17386101]. It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but function in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling [PMID:15927959,11959810,11283269].
|Product Specific Protocols|
|WB protocol for ARID3A antibody 14068-1-AP||Download protocol|
|IHC protocol for ARID3A antibody 14068-1-AP||Download protocol|
|IF protocol for ARID3A antibody 14068-1-AP||Download protocol|
|IP protocol for ARID3A antibody 14068-1-AP||Download protocol|
|Click here to view our Standard Protocols|
ARID3A Positivity Correlated With Favorable Prognosis in Patients With Residual Rectal Cancer After Neoadjuvant Chemoradiotherapy.
Ann Surg Oncol
High Expression of AT-Rich Interactive Domain 3A (ARID3A) is Associated with Good Prognosis in Colorectal Carcinoma.
ARID3A promotes the development of colorectal cancer by upregulating AURKA.
Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2.