ARID3A Antibody

ARID3A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14068-1-AP

Synonyms

ARID3A, BRIGHT, Dead ringer like protein 1, DRIL1, DRIL3, DRX, E2F binding protein 1, E2FBP1



Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14068-1-AP targets ARID3A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARID3A fusion protein Ag5124
Full Name AT rich interactive domain 3A (BRIGHT-like)
Calculated molecular weight 63 kDa
Observed molecular weight 75 kDa
GenBank accession number BC060828
Gene symbol ARID3A
Gene ID (NCBI) 1820
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) [PMID:17386101]. It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but function in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling [PMID:15927959,11959810,11283269].

Protocols

Product Specific Protocols
WB protocol for ARID3A antibody 14068-1-APDownload protocol
IHC protocol for ARID3A antibody 14068-1-APDownload protocol
IF protocol for ARID3A antibody 14068-1-APDownload protocol
IP protocol for ARID3A antibody 14068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Anticancer Res

ARID3A Positivity Correlated With Favorable Prognosis in Patients With Residual Rectal Cancer After Neoadjuvant Chemoradiotherapy.

Authors - Ghilsuk Yoon
humanWB,IHC

Ann Surg Oncol

High Expression of AT-Rich Interactive Domain 3A (ARID3A) is Associated with Good Prognosis in Colorectal Carcinoma.

Authors - Meiying Song
mouseIHC

PLoS Genet

Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2.

Authors - Blair B Madison