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Arrestin C Polyclonal antibody

Arrestin C Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11100-2-AP

Synonyms

ARR3, Arrestin C, ARRX, C arrestin, CAR, cArr, Cone arrestin, Retinal cone arrestin 3, X arrestin

Validation Data Gallery

  • WB analysis of human brain using 11100-2-AP

    WB analysis of human brain using 11100-2-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11100-2-AP (Arrestin C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 11100-2-AP

    IHC staining of mouse eye using 11100-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11100-2-AP (Arrestin C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 11100-2-AP

    IHC staining of mouse eye using 11100-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11100-2-AP (Arrestin C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue, mouse eye tissue, mouse heart tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

11100-2-AP targets Arrestin C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Arrestin C fusion protein Ag1580
Full Name arrestin 3, retinal (X-arrestin)
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC012096
Gene symbol ARR3
Gene ID (NCBI) 407
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Arrestin C antibody 11100-2-APDownload protocol
IHC protocol for Arrestin C antibody 11100-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

Tridimensional Retinoblastoma Cultures as Vitreous Seeds Models for Live-Cell Imaging of Chemotherapy Penetration.

Authors - Ursula Winter
View Article
humanIHC

Cancers (Basel)

Clinical, Genomic, and Pharmacological Study of MYCN-Amplified RB1 Wild-Type Metastatic Retinoblastoma.

Authors - Santiago Zugbi
View Article