Tested Applications
| Positive WB detected in | L02 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue |
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25616-1-AP targets ASB13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22533 Product name: Recombinant human ASB13 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 5-173 aa of BC012056 Sequence: AADGCFLGDVGFWVERTPVHEAAQRGESLQLQQLIESGACVNQVTVDSITPLHAASLQGQARCVQLLLAAGAQVDARNIDGSTPLCDACASGSIECVKLLLSYGAKVNPPLYTASPLHEACMSGSSECVRLLIDVGANLEAHDCHFGTPLHVACAREHLDCVKVLLNAA Predict reactive species |
| Full Name | ankyrin repeat and SOCS box-containing 13 |
| Calculated Molecular Weight | 278 aa, 30 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC012056 |
| Gene Symbol | ASB13 |
| Gene ID (NCBI) | 79754 |
| RRID | AB_2880162 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXK3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ASB13 antibody 25616-1-AP | Download protocol |
| IHC protocol for ASB13 antibody 25616-1-AP | Download protocol |
| WB protocol for ASB13 antibody 25616-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ASB13 Polyclonal antibody (Cat# 25616-1-AP) has 1 total citation. It was cited in ACS Omega (Impact Factor 5.2, 2025), in a study on novel biomarkers for lymph node metastasis and prognosis of bladder cancer using bioinformatics analysis. The cited application is IHC in human samples, placing the research field in oncology and bladder cancer biomarker discovery.
| Species | Application | Title |
|---|---|---|
ACS Omega Novel Biomarkers for Lymph Node Metastasis and Prognosis of Bladder Cancer by Bioinformatics Analysis. |
