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ASB13 Polyclonal antibody

ASB13 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25616-1-AP

Synonyms

ASB 13, ASB13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ASB13 Polyclonal antibody (25616-1-AP)

    WB analysis using 25616-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25616-1-AP (ASB13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ASB13 Polyclonal antibody (25616-1-AP)

    IHC staining of human small intestine using 25616-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25616-1-AP (ASB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ASB13 Polyclonal antibody (25616-1-AP)

    IHC staining of human colon using 25616-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25616-1-AP (ASB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ASB13 Polyclonal antibody (25616-1-AP)

    IHC staining of human colon using 25616-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25616-1-AP (ASB13 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ASB13 Polyclonal antibody (25616-1-AP)

    IHC staining of human colon using 25616-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25616-1-AP (ASB13 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ASB13 Polyclonal antibody (25616-1-AP)

    IHC staining of human small intestine using 25616-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25616-1-AP (ASB13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ASB13 Polyclonal antibody (25616-1-AP)

    IF Staining of HepG2 using 25616-1-AP

    Immunofluorescent analysis of HepG2 cells using 25616-1-AP (ASB13 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inL02 cells, mouse heart tissue, mouse liver tissue, rat heart tissue, rat liver tissue
Positive IHC detected inhuman small intestine tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25616-1-AP is ASB13 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASB13 fusion protein Ag22533
Full Name ankyrin repeat and SOCS box-containing 13
Calculated Molecular Weight 278 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC012056
Gene Symbol ASB13
Gene ID (NCBI) 79754
RRIDAB_2880162
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ASB13 antibody 25616-1-APDownload protocol
IHC protocol for ASB13 antibody 25616-1-APDownload protocol
IF protocol for ASB13 antibody 25616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols