ASB13 Polyclonal antibody

ASB13 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25616-1-AP

Synonyms

ASB 13, ASB13



Tested Applications

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IHC detected inhuman heart tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25616-1-AP is ASB13 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASB13 fusion protein Ag22533
Full Name ankyrin repeat and SOCS box-containing 13
Calculated molecular weight 278 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC012056
Gene symbol ASB13
Gene ID (NCBI) 79754
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ASB13 antibody 25616-1-APDownload protocol
IHC protocol for ASB13 antibody 25616-1-APDownload protocol
IF protocol for ASB13 antibody 25616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols