• Featured Product
  • KD/KO Validated

ASNS Polyclonal antibody

ASNS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14681-1-AP

Synonyms

ASNS, asparagine synthetase, TS11



Tested Applications

Positive WB detected inJurkat cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman retinoblastoma tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14681-1-AP targets ASNS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASNS fusion protein Ag6393
Full Name asparagine synthetase
Calculated molecular weight 64 kDa
Observed molecular weight 55-64 kDa
GenBank accession numberBC014621
Gene symbol ASNS
Gene ID (NCBI) 440
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.

Protocols

Product Specific Protocols
WB protocol for ASNS antibody 14681-1-APDownload protocol
IHC protocol for ASNS antibody 14681-1-APDownload protocol
IF protocol for ASNS antibody 14681-1-APDownload protocol
IP protocol for ASNS antibody 14681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

PMID: 30996100

Authors - Anil Pant
  • KD Validated
humanWB,IHC

Nat Med

The landscape of cancer cell line metabolism.

Authors - Haoxin Li
  • KD Validated
humanWB

Cell Rep

Liver-Type Glutaminase GLS2 Is a Druggable Metabolic Node in Luminal-Subtype Breast Cancer.

Authors - Michael J Lukey
humanWB

MBio

Asparagine Deprivation Causes a Reversible Inhibition of Human Cytomegalovirus Acute Virus Replication.

Authors - Chen-Hsuin Lee
  • KD Validated
humanWB

Chem Biol Drug Des

Down-regulation of asparagine synthetase induces cell cycle arrest and inhibits cell proliferation of breast cancer.

Authors - Hongjian Yang
  • KD Validated
humanWB

Biotechnol Appl Biochem

Knockdown of asparagine synthetase by RNAi suppresses cell growth in human melanoma cells and epidermoid carcinoma cells.

Authors - Hui Li
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (12-02-2019)

Human RCC4 kidney cancer cell lysate was denatured in SDS-sample buffer and size-separated on a denaturing gel. A specific band appeared for ASNS.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human kidney cancer cells (RCC4)
ASNS Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:14681-1-AP)
FH

Daniel (Verified Customer) (12-02-2019)

Mouse kidney stained for Asns (green, AF488) and nucleus (blue, DAPI).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse kidney
ASNS Antibody Immunofluorescence, validation (1:200 dilution) in Mouse kidney (Cat no:14681-1-AP)