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ATF1 Antibody

ATF1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11946-1-AP

Synonyms

ATF1, EWS ATF1, FUS/ATF 1, Protein TREB36, TREB36

Validation Data Gallery

  • ATF1 Antibody WB HeLa cells 11946-1-AP

    WB analysis of HeLa cells using 11946-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11946-1-AP(ATF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

  • ATF1 Antibody IP HeLa cells 11946-1-AP

    IP experiment of HeLa cells using 11946-1-AP

    IP Result of anti-ATF1 (IP:11946-1-AP, 4ug; Detection:11946-1-AP 1:500) with HeLa cells lysate 3200ug.

  • ATF1 Antibody IF HeLa cells 11946-1-AP

    IF staining of HeLa cells using 11946-1-AP

    Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11946-1-AP(ATF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Tested Applications

Positive WB detected inHeLa cells, A431 cells, human brain tissue
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11946-1-AP targets ATF1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATF1 fusion protein Ag2548
Full Name activating transcription factor 1
Calculated molecular weight 271aa,29 kDa
Observed molecular weight 35 kDa
GenBank accession number BC029619
Gene symbol ATF1
Gene ID (NCBI) 466
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATF1 antibody 11946-1-APDownload protocol
IF protocol for ATF1 antibody 11946-1-APDownload protocol
IP protocol for ATF1 antibody 11946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cells

Perilipin 5 Protects against Cellular Oxidative Stress by Enhancing Mitochondrial Function in HepG2 Cells.

Authors - Yanjie Tan
View Article
humanWB

J Biol Chem

The mRNA levels of heat shock factor 1 are regulated by thermogenic signals via the cAMP-dependent transcription factor ATF3.

Authors - Narendra Verma
View Article
humanWB

Am J Hum Genet

Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction.

Authors - Jianbo Tian
  • KD Validated
View Article