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  • Featured Product
  • KD/KO Validated

ATF1 Polyclonal antibody

ATF1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11946-1-AP

Synonyms

ATF1, EWS ATF1, FUS/ATF 1, Protein TREB36, TREB36

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ATF1 Polyclonal antibody (11946-1-AP)

    WB analysis of HeLa using 11946-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ATF1 Polyclonal antibody (11946-1-AP)

    WB analysis of human brain using 11946-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using ATF1 Polyclonal antibody (11946-1-AP)

    WB analysis of A431 using 11946-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11946-1-AP (ATF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ATF1 Polyclonal antibody (11946-1-AP)

    IP experiment of HeLa using 11946-1-AP

    IP result of anti-ATF1 (IP:11946-1-AP, 4ug; Detection:11946-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATF1 Polyclonal antibody (11946-1-AP)

    IF Staining of HeLa using 11946-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11946-1-AP (ATF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A431 cells, human brain tissue
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11946-1-AP targets ATF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATF1 fusion protein Ag2548
Full Name activating transcription factor 1
Calculated Molecular Weight 271 aa, 29 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC029619
Gene Symbol ATF1
Gene ID (NCBI) 466
RRIDAB_2060777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFs play a vital role in maintaining cell homeostasis by combining DNA regulatory sequences to regulate gene transcription rates. Activating transcription factor 1 (ATF1) is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site 'TGACGTCA'(PMID: 28032861). ATF1 transcriptionally regulates the expressions of target genes to mediate various cellular processes, and ATF1 effects the progression of tumors (including lung cancer, colorectal cancer,and nasopharyngeal carcinoma).

Protocols

Product Specific Protocols
WB protocol for ATF1 antibody 11946-1-APDownload protocol
IF protocol for ATF1 antibody 11946-1-APDownload protocol
IP protocol for ATF1 antibody 11946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Hum Genet

Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction.

Authors - Jianbo Tian
  • KD Validated
View Article
mouseWB

Mol Ther Nucleic Acids

Mbd2 deficiency alleviates retinal cell apoptosisvia the miR-345-5p/Atf1 axis in high glucoseinjury and streptozotocin-induced diabetic mice.

Authors - Yanni Ge
View Article
humanWB

Sci Signal

The death-inducing activity of RIPK1 is regulated by the pH environment.

Authors - Kenta Moriwaki
View Article
humanWB

Front Cell Dev Biol

CREB1 and ATF1 Negatively Regulate Glutathione Biosynthesis Sensitizing Cells to Oxidative Stress.

Authors - Lina Zhao
  • KD Validated
View Article
humanWB

J Biol Chem

The mRNA levels of heat shock factor 1 are regulated by thermogenic signals via the cAMP-dependent transcription factor ATF3.

Authors - Narendra Verma
View Article
humanWB

Cells

Perilipin 5 Protects against Cellular Oxidative Stress by Enhancing Mitochondrial Function in HepG2 Cells.

Authors - Yanjie Tan
View Article