Tested Applications
| Positive WB detected in | mouse spleen tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24322-1-AP targets ATG16L2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19399 Product name: Recombinant human ATG16L2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 17-365 aa of BC137490 Sequence: HIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALRVKWQEEEEGLRLVCGEMAYQVVEKGAALGTLESELQQRQSRLAALEARVAQLREARAQQAQQVEEWRAQNAVQRAAYEALRAHVGLREAALRRLQEEARDLLERLVQRKARAAAERNLRNERRERAKQARVSQELKKAAKRTVSISEGPDTLGDGMRERRETLALAPEPEPLEKEACEKWKRPFRSASATSLTLSHCVDVVKGLLDFKKRRGHSIGGAPEQRYQIIPVCVAARLPTRAQDVLDAHLSEVNAVRFGPNSSLLATGGADRLIHLWNV Predict reactive species |
| Full Name | ATG16 autophagy related 16-like 2 (S. cerevisiae) |
| Calculated Molecular Weight | 619 aa, 69 kDa |
| Observed Molecular Weight | 70 kDa, 30 kDa |
| GenBank Accession Number | BC137490 |
| Gene Symbol | ATG16L2 |
| Gene ID (NCBI) | 89849 |
| RRID | AB_2879493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NAA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATG16L2 antibody 24322-1-AP | Download protocol |
| IP protocol for ATG16L2 antibody 24322-1-AP | Download protocol |
| WB protocol for ATG16L2 antibody 24322-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATG16L2 Polyclonal antibody (Cat# 24322-1-AP) has 1 total citation. It appears in the journal Gene (IF 2.7), published in May 2022. The cited study investigated intron retention as a stress response in sensor genes and its restoration by Japanese herbal medicines, using the antibody for Western blotting in mouse samples. The research field is gene regulation and stress response biology.
| Species | Application | Title |
|---|---|---|
Gene Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications. |
