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- KD/KO Validated
ATP1A1-Specific Polyclonal antibody
ATP1A1-Specific Polyclonal Antibody for FC, IF, IHC, WB,ELISA
Cat no : 55187-1-AP
|Positive WB detected in||HEK-293 cells, mouse heart tissue, Neuro-2a cells, MCF-7 cells, HepG2 cells|
|Positive IHC detected in||human liver cancer tissue, mouse kidney tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Caco-2 cells, HEK-293 cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55187-1-AP targets ATP1A1-Specific in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||ATPase, Na+/K+ transporting, alpha 1 polypeptide|
|Calculated molecular weight||113 kDa|
|Observed molecular weight||100-110 kDa|
|GenBank accession number||NM_000701|
|Gene ID (NCBI)||476|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.
|Product Specific Protocols|
|WB protocol for ATP1A1-Specific antibody 55187-1-AP||Download protocol|
|IHC protocol for ATP1A1-Specific antibody 55187-1-AP||Download protocol|
|IF protocol for ATP1A1-Specific antibody 55187-1-AP||Download protocol|
|FC protocol for ATP1A1-Specific antibody 55187-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Identification of the retinoschisin-binding site on the retinal Na/K-ATPase.
Metab Brain Dis
The metabolic changes in the hippocampus of an atherosclerotic rat model and the regulation of aerobic training.
A Novel Bufalin Derivative Exhibited Stronger Apoptosis-Inducing Effect than Bufalin in A549 Lung Cancer Cells and Lower Acute Toxicity in Mice.
Mol Biol Cell
Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.