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ATP1A1-Specific Polyclonal antibody

ATP1A1-Specific Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 55187-1-AP

Synonyms

ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1



Tested Applications

Positive WB detected inHEK-293 cells, mouse heart tissue, Neuro-2a cells, MCF-7 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells, HEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55187-1-AP targets ATP1A1-Specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated molecular weight 113 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberNM_000701
Gene symbol ATP1A1
Gene ID (NCBI) 476
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.

Protocols

Product Specific Protocols
WB protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
IHC protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
IF protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
FC protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Identification of the retinoschisin-binding site on the retinal Na/K-ATPase.

Authors - Karolina Plössl
ratWB

Metab Brain Dis

The metabolic changes in the hippocampus of an atherosclerotic rat model and the regulation of aerobic training.

Authors - Beibei Liu
humanWB

PLoS One

A Novel Bufalin Derivative Exhibited Stronger Apoptosis-Inducing Effect than Bufalin in A549 Lung Cancer Cells and Lower Acute Toxicity in Mice.

Authors - Miao Liu
mouseIF

Mol Biol Cell

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Authors - Karolina Plössl