Tested Applications
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-55187 targets ATP1A1-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ATPase, Na+/K+ transporting, alpha 1 polypeptide |
| Calculated Molecular Weight | 113 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | NM_000701 |
| Gene Symbol | ATP1A1 |
| Gene ID (NCBI) | 476 |
| ENSEMBL Gene ID | ENSG00000163399 |
| RRID | AB_3084127 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05023 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 ATP1A1-Specific antibody CL488-55187 | Download protocol |
| IF protocol for CL Plus 488 ATP1A1-Specific antibody CL488-55187 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The user performed immunofluorescence on MDA-MB-231 cells using a 1:100 dilution and reported that the antibody works really well, effectively labeling cell membranes without background signal due to the secondary antibody. Overall, the feedback highlights strong performance and clean labeling in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Paulina (Verified Customer) (04-22-2025) | Works really well, labels cell membranes effectively without background due to secondary antibody
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