ATP1B1 Polyclonal antibody

ATP1B1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15192-1-AP

Synonyms

ATP1B, ATP1B1, Beta 1 subunit of Na(+) K(+) ATPase, Na+/K+ ATPase beta 1 subunit



Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, human brain tissue, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15192-1-AP targets ATP1B1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP1B1 fusion protein Ag7279
Full Name ATPase, Na+/K+ transporting, beta 1 polypeptide
Calculated molecular weight 35 kDa
Observed molecular weight 49-52 kDa
GenBank accession numberBC000006
Gene symbol ATP1B1
Gene ID (NCBI) 481
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)

Protocols

Product Specific Protocols
WB protocol for ATP1B1 antibody 15192-1-APDownload protocol
IHC protocol for ATP1B1 antibody 15192-1-APDownload protocol
IF protocol for ATP1B1 antibody 15192-1-APDownload protocol
IP protocol for ATP1B1 antibody 15192-1-APDownload protocol
FC protocol for ATP1B1 antibody 15192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells.

Authors - Shu-Hong Zhang
human

PLoS One

Identification of the retinoschisin-binding site on the retinal Na/K-ATPase.

Authors - Karolina Plössl
mouseWB

Obesity (Silver Spring)

27-Hydroxycholesterol Inhibits Sterol Regulatory Element-Binding Protein 1 Activation and Hepatic Lipid Accumulation in Mice.

Authors - Di Li
ratWB

Int J Mol Sci

RBM20 Regulates CaV1.2 Surface Expression by Promoting Exon 9* Inclusion of CACNA1C in Neonatal Rat Cardiomyocytes.

Authors - Akihito Morinaga
mouseIF

Mol Biol Cell

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Authors - Karolina Plössl