|Positive WB detected in||mouse brain tissue, human heart tissue, human brain tissue, mouse heart tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human brain tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15192-1-AP targets ATP1B1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP1B1 fusion protein Ag7279|
|Full Name||ATPase, Na+/K+ transporting, beta 1 polypeptide|
|Calculated molecular weight||35 kDa|
|Observed molecular weight||49-52 kDa|
|GenBank accession number||BC000006|
|Gene ID (NCBI)||481|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ATP1B1 is one of beta subunits of the Na+/K+ ATPase and responsible for formation and structural integrity of the Na+/K+ ATPase. The Na+/K+ ATPase is a plasma membrane pump consisting of alpha, beta, and gamma subunits. At least four of Na+/K+-ATPase beta subunits (β1, β2, β3, β4) have been identified in mammalian cells; the β1-subunit (ATP1B1) is the most ubiquitous. The Na+/K+ ATPase β subunits have multiple N-glycosylation sites. The predicted MW of ATP1B1 is 35 kDa, while it migrates around 40-52 kDa due to the variable glycosylation. (PMID: 10896885, 17714085)
|Product Specific Protocols|
|WB protocol for ATP1B1 antibody 15192-1-AP||Download protocol|
|IHC protocol for ATP1B1 antibody 15192-1-AP||Download protocol|
|IF protocol for ATP1B1 antibody 15192-1-AP||Download protocol|
|IP protocol for ATP1B1 antibody 15192-1-AP||Download protocol|
|FC protocol for ATP1B1 antibody 15192-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.