ATP5A1 Monoclonal antibody

ATP5A1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10H3

Cat no : 66037-1-Ig

Synonyms

ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, MOM2, OMR, ORM



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, HepG2 cells, HEK-293 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:150-1:600
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66037-1-Ig is ATP5A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ATP5A1 fusion protein Ag8119
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Calculated molecular weight 60 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC064562
Gene symbol ATP5A1
Gene ID (NCBI) 498
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ATP5A1 antibody 66037-1-IgDownload protocol
IHC protocol for ATP5A1 antibody 66037-1-IgDownload protocol
IF protocol for ATP5A1 antibody 66037-1-IgDownload protocol
IP protocol for ATP5A1 antibody 66037-1-IgDownload protocol
FC protocol for ATP5A1 antibody 66037-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

Succinate-induced neuronal mitochondrial fission and hexokinase II malfunction in ischemic stroke: Therapeutical effects of kaempferol.

Authors - Bin Wu
mouseWB

Proteomics

Quantitative Proteomic Study of Myocardial Mitochondria in Urea Transporter B Knockout Mice.

Authors - Yanwei Du
humanIHC

Sci Rep

Region of Interest analysis using mass spectrometry imaging of mitochondrial and sarcomeric proteins in acute cardiac infarction tissue.

Authors - Yuka Yajima
mouseIF

Oncotarget

Ginkgolide K attenuates neuronal injury after ischemic stroke by inhibiting mitochondrial fission and GSK-3β-dependent increases in mitochondrial membrane permeability.

Authors - Xu Zhou

Cell Death Dis

Succinate induces aberrant mitochondrial fission in cardiomyocytes through GPR91 signaling.

Authors - Yi-Tong Lu
humanWB

Autophagy

AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Authors - Zhenhong Ni