ATP5A1 Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66037-1-Ig |CloneNo.: 1B10H3

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Freight/Packing

Con: 142 μg/150 μl

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Species specificity:
human, mouse, rat, monkey

Positive WB detected in:
multi-cells, HeLa cells, MCF-7 cells

Positive IP detected in:
mouse heart tissue

Positive IHC detected in:
human heart tissue, human heart tissue, human liver tissue

Positive IF detected in:
Hela cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle

Calculated molecular weight:
60 kDa

Observed molecular weight:
50 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ATP5A1

Synonyms
ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, MOM2, OMR, ORM
Background

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues.


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