CoraLite®488-conjugated ATP5A1 Monoclonal antibody

ATP5A1 Monoclonal Antibody for FC, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, monkey

Applications

IF, FC, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1B10H3

Cat no : CL488-66037

Synonyms

ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, MOM2, OMR, ORM



Tested Applications

Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66037-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66037 is ATP5A1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ATP5A1 fusion protein Ag8119
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Calculated molecular weight 60 kDa
GenBank accession numberBC064562
Gene symbol ATP5A1
Gene ID (NCBI) 498
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3..
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated ATP5A1 antibody CL488-66037Download protocol
FC protocol for CL488-conjugated ATP5A1 antibody CL488-66037Download protocol
Standard Protocols
Click here to view our Standard Protocols