CoraLite®555-conjugated ATP5A1 Monoclonal antibody

ATP5A1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, monkey

Applications

IF, FC (Intra), FC

Conjugate

CoraLite®555 Fluorescent Dye

Publications

1

CloneNo.

1B10H3

Cat no : CL555-66037

Synonyms

ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM

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Validation Data Gallery

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  IF Staining of HepG2 using CL555-66037

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®555 ATP5A1 antibody (CL555-66037, Clone: 1B10H3 ) at dilution of 1:200, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

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  FC experiment of HeLa using CL555-66037

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®555 Anti-Human ATP5A1 (CL555-66037, Clone:1B10H3) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

The immunogen of CL555-66037 is ATP5A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATP5A1 fusion protein Ag8119
• Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC064562
• Gene Symbol: ATP5A1
• Gene ID (NCBI): 498
• RRID: AB_2919670
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Publications

Species	Application	Title
mouse	FC	Nat Immunol; Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis; Authors - Yavuz F Yazicioglu

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C