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ATP6V1B1 Polyclonal antibody

ATP6V1B1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14780-1-AP

Synonyms

ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using ATP6V1B1 Polyclonal antibody (14780-1-AP)

    WB analysis of mouse kidney using 14780-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14780-1-AP (ATP6V1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ATP6V1B1 Polyclonal antibody (14780-1-AP)

    WB analysis of human brain using 14780-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14780-1-AP (ATP6V1B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6V1B1 Polyclonal antibody (14780-1-AP)

    IHC staining of human kidney using 14780-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14780-1-AP (ATP6V1B1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ATP6V1B1 Polyclonal antibody (14780-1-AP)

    IHC staining of human kidney using 14780-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14780-1-AP (ATP6V1B1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP6V1B1 Polyclonal antibody (14780-1-AP)

    IF Staining of HEK-293 using 14780-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP6V1B1 antibody (14780-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse kidney tissue, human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14780-1-AP targets ATP6V1B1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1B1 fusion protein Ag6332
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC063411
Gene Symbol ATP6V1B1
Gene ID (NCBI) 525
RRIDAB_2062378
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of1 ATP6V1B1 is 57 kDa.

Protocols

Product Specific Protocols
WB protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
IHC protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
IF protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Life Sci

The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.

Authors - Enrico Castroflorio
View Article
mouseIF

Dis Model Mech

Expression of N471D strumpellin leads to defects in the endolysosomal system.

Authors - Lin Song
View Article
humanWB

J Cell Physiol

Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts.

Authors - Milena Kluge
View Article