ATP6V1B1 Polyclonal antibody

ATP6V1B1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14780-1-AP

Synonyms

ATP6B1, ATP6V1B1, RTA1B, V ATPase subunit B 1, VATB, VMA2, VPP3



Tested Applications

Positive WB detected inhuman kidney tissue, human brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14780-1-AP targets ATP6V1B1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1B1 fusion protein Ag6332
Full Name ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
Calculated molecular weight 57 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC063411
Gene symbol ATP6V1B1
Gene ID (NCBI) 525
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
IHC protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
IF protocol for ATP6V1B1 antibody 14780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-β1 in salivary ducts.

Authors - Milena Kluge
mouseIF

Dis Model Mech

Expression of N471D strumpellin leads to defects in the endolysosomal system.

Authors - Lin Song