ATP6V1E2 Polyclonal antibody

ATP6V1E2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13235-1-AP

Synonyms

ATP6E1, ATP6EL2, ATP6V1E2, ATP6V1EL2, V ATPase subunit E 2, VMA4



Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13235-1-AP targets ATP6V1E2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1E2 fusion protein Ag4088
Full Name ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2
Calculated Molecular Weight 226 aa, 26 kDa
Observed Molecular Weight 31-33 kDa
GenBank Accession NumberBC034808
Gene Symbol ATP6V1E2
Gene ID (NCBI) 90423
RRIDAB_2062675
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ATP6V1E2 antibody 13235-1-APDownload protocol
IP protocol for ATP6V1E2 antibody 13235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols