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ATXN3L Polyclonal antibody

ATXN3L Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 23166-1-AP

Synonyms

ataxin 3 like, ATX3L, ATXN3L, MJDL, Putative ataxin 3 like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using ATXN3L Polyclonal antibody (23166-1-AP)

    WB analysis of human testis using 23166-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 23166-1-AP (ATXN3L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using ATXN3L Polyclonal antibody (23166-1-AP)

    WB analysis of human testis using 23166-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 23166-1-AP (ATXN3L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ATXN3L Polyclonal antibody (23166-1-AP)

    IHC staining of human testis using 23166-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23166-1-AP (ATXN3L Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ATXN3L Polyclonal antibody (23166-1-AP)

    IHC staining of human testis using 23166-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23166-1-AP (ATXN3L Antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23166-1-AP is ATXN3L Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATXN3L fusion protein Ag19611
Full Name ataxin 3-like
Calculated Molecular Weight 355 aa, 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC137187
Gene Symbol ATXN3L
Gene ID (NCBI) 92552
RRIDAB_2879221
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN3L, also named as Ataxin-3-like protein, is a 355 amino acid protein, which contains 1 Josephin domain and 2 UIM (ubiquitin-interacting motif) domains. ATXN3L localizes in the nucleus and as a deubiquitinating enzyme that cleaves both 'Lys-48'-linked and 'Lys-63'-linked poly-ubiquitin chains.

Protocols

Product Specific Protocols
WB protocol for ATXN3L antibody 23166-1-APDownload protocol
IHC protocol for ATXN3L antibody 23166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols