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AVEN Polyclonal antibody

AVEN Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25846-1-AP

Synonyms

AVEN, Cell death regulator Aven, PDCD12

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using AVEN Polyclonal antibody (25846-1-AP)

    WB analysis of MCF-7 using 25846-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25846-1-AP (AVEN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using AVEN Polyclonal antibody (25846-1-AP)

    WB analysis of K-562 using 25846-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25846-1-AP (AVEN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMCF-7 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 25846-1-AP is AVEN Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AVEN fusion protein Ag23020
Full Name apoptosis, caspase activation inhibitor
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC010488
Gene Symbol AVEN
Gene ID (NCBI) 57099
RRIDAB_2880266
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AVEN (Apoptosis caspase activation inhibitor) is known to play a role in male and female germ cell development. AVEN protein has also been shown to inhibit the mitochondrial apoptosis pathway by binding to and inhibiting the self-association of proapoptotic APAF-1, as well as binding to and enhancing antiapoptotic BCL-xL activity. AVEN has also recently been shown to function as an activator of the cell cycle-regulating ATM kinase in the DNA damage response pathway. Molecular weight of this protein detected by WB is 50 kDa (PMID: 22751129).

Protocols

Product Specific Protocols
WB protocol for AVEN antibody 25846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Open Med (Wars)

Tanshinone IIA reverses oxaliplatin resistance in colorectal cancer through microRNA-30b-5p/AVEN axis.

Authors - Tingrui Ge
View Article
humanWB

Front Oncol

Integrative proteomics and m6A microarray analyses of the signatures induced by METTL3 reveals prognostically significant in gastric cancer by affecting cellular metabolism

Authors - Guisen Peng
View Article