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AXL Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 13196-1-AP

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Con: 32 μg/150 μl

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Tested Applications

Applications:
WB, IP, FC, ELISA
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Images See all

AXL Antibody WB HepG2 cells 13196-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 13196-1-AP(AXL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

AXL Antibody IP HepG2 cells 13196-1-AP

IP Result of anti-AXL (IP:13196-1-AP, 5ug; Detection:13196-1-AP 1:1000) with HepG2 cells lysate 9000ug.

AXL Antibody FC HeLa cells 13196-1-AP

1X10^6 HeLa cells were stained with 0.2ug AXL antibody (13196-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, HeLa cells, HT-1080 cells, human liver tissue
Positive IP detected in:
HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AXL fusion protein Ag3898
Full name:
AXL receptor tyrosine kinase
Calculated molecular weight:
894aa,98 kDa
Observed molecular weight:
140 kDa
GenBank accession number:
BC032229
Gene ID (NCBI):
558
Gene symbol
AXL
Synonyms
AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO

Background

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. This antibody detects a single band at 140 kDa, which is considered to represent the auto-phosphorylated form of endogenous Axl (PMID: 12730092).


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