HeLa cells were subjected to SDS PAGE followed by western blot with 13196-1-AP(AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 13196-1-AP(AXL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

HT-1080 cells were subjected to SDS PAGE followed by western blot with 13196-1-AP(AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

human liver tissue were subjected to SDS PAGE followed by western blot with 13196-1-AP(AXL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IP Result of anti-AXL (IP:13196-1-AP, 5ug; Detection:13196-1-AP 1:1000) with HepG2 cells lysate 9000ug.

1X10^6 HeLa cells were stained with 0.2ug AXL antibody (13196-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.