AXL Polyclonal antibody

AXL Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13196-1-AP

Synonyms

AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HT-1080 cells, human liver tissue
Positive IP detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

13196-1-AP targets AXL in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AXL fusion protein Ag3898
Full Name AXL receptor tyrosine kinase
Calculated molecular weight 894 aa, 98 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC032229
Gene symbol AXL
Gene ID (NCBI) 558
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. This antibody detects a single band at 140 kDa, which is considered to represent the auto-phosphorylated form of endogenous Axl (PMID: 12730092).

Protocols

Product Specific Protocols
WB protocol for AXL antibody 13196-1-APDownload protocol
IP protocol for AXL antibody 13196-1-APDownload protocol
FC protocol for AXL antibody 13196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Eur J Pharmacol

PMID: 27212383

Authors - Xiaoyu Jiang
ratWB

Mol Cell Biochem

Vitamin K2 inhibits rat vascular smooth muscle cell calcification by restoring the Gas6/Axl/Akt anti-apoptotic pathway.

Authors - Cuiting Qiu
humanWB, IHC,IF

Cancer Gene Ther

Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.

Authors - Minhai Dong
humanWB

Int J Cancer

Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.

Authors - Yingying Shen