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Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody (82828-14-RR)

Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody

Acetyl-Histone H3 (Lys36) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR

Cat No. 82828-14-RR

Clone No. 250370A9

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR

Synonyms

Ac Histone H3, Ac Histone H3 (Lys36), Ac Histone H3 Lys36, Ac Histone H3-Lys36, Acetyl Histone

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Quantity

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Filter: AllWBIF/ICCFC (Intra)Dot BlotChIP-qPCR

WB analysis using 82828-14-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82828-14-RR (Acetyl-Histone H3 (Lys36) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of NIH/3T3 using 82828-14-RR
Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated NIH/3T3 cells using Acetyl-Histone H3 (Lys36) antibody (82828-14-RR, Clone: 250370A9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 82828-14-RR
1X10^6 HeLa cells untreated (dashed lines) or treated with Trichostatin A treated which intracellularly stained with 0.13 ug Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody (82828-14-RR, Clone:250370A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1X10^6 HeLa cells untreated (dashed lines) or treated with Trichostatin A treated which intracellularly stained with 0.13 ug Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody (82828-14-RR, Clone:250370A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
FC experiment of NIH/3T3 using 82828-14-RR
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Trichostatin A treated which intracellularly stained with 0.13 ug Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody (82828-14-RR, Clone:250370A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Trichostatin A treated which intracellularly stained with 0.13 ug Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody (82828-14-RR, Clone:250370A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
Dot Blot experiment using 82828-14-RR
Dot blot analysis was used to confirm the specificity of 82828-14-RR Acetyl-Histone H3(Lys36) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
ChIP experiment of HeLa using 82828-14-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of HIST1H3A (82828-14-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Acetyl-Histone H3 (Lys36) in ?

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Trichostatin A treated NIH/3T3 cells
Positive IF/ICC detected in	Trichostatin A treated NIH/3T3 cells
Positive FC (Intra) detected in	Trichostatin A treated HeLa cells, Trichostatin A treated NIH/3T3 cells
Positive Dot Blot detected in	N/A N/A
Positive ChIP-qPCR detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
DOT BLOT	DOT BLOT : 1:10-1:100
CHIP-QPCR	CHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82828-14-RR targets Acetyl-Histone H3 (Lys36) in WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	histone cluster 1, H3a
Observed Molecular Weight	15 kDa
GenBank Accession Number	BC066245
Gene Symbol	HIST1H3A
Gene ID (NCBI)	8350
RRID	AB_3743437
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P68431
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

H3K36ac peaks at the 5' end of genes,manily on the two nucleosomeus immediately distal to the transcription start site, independently of gene length. H3K36ac and H3K36mac showed negative creastalk, which is mediated by GCN3 and the histone methyl transferase SDX8. Although H3K36ac is associated with gene activity, there is no linear relationship between H3K36ac and transcript levels, suggesting that H3K36ac is a binary indicator of transcription.

Protocols

Product Specific Protocols
FC protocol for Acetyl-Histone H3 (Lys36) antibody 82828-14-RR	Download protocol
IF protocol for Acetyl-Histone H3 (Lys36) antibody 82828-14-RR	Download protocol
WB protocol for Acetyl-Histone H3 (Lys36) antibody 82828-14-RR	Download protocol