Beta Amyloid Polyclonal antibody

Beta Amyloid Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25524-1-AP

Synonyms

A4, AAA, ABETA, ABPP, AD1, AICD 50, AICD 57, AICD 59, AID(50), AID(57), AID(59), amyloid beta 1-42, Amyloid beta A4 protein, amyloid P, Amyloid-beta protein 40, Amyloid-beta protein 42, APP, APPI, Aβ1-42, beta Amyloid, C31, C80, C83, C99, CTFgamma, CVAP, Gamma CTF(50) C31, Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59,, P3(40), PN II, PN2, PreA4, Protease nexin II, S APP alpha Soluble APP beta



Tested Applications

Positive WB detected inSH-SY5Y cells, 3T3-L1 cells, HeLa cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25524-1-AP is Beta Amyloid Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Amyloid fusion protein Ag22408
Full Name amyloid beta (A4) precursor protein
Observed molecular weight 100 kDa
GenBank accession numberBC065529
Gene symbol APP
Gene ID (NCBI) 351
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. This antibody can recognize several isoforms of both mature and immature amyloid beta (A4) precursor protein, including APP770, APP677, APP695, APP696, APP733, APP751, APP752, and APP639. APP can be cleaved into several chains, this antibody could recognize fragments C99, Amyloid-beta protein 42, Amyloid-beta protein 40, C83, P3(40), C80, Gamma-secretase C-terminal fragment 59, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 50, C31.

Protocols

Product Specific Protocols
WB protocol for Beta Amyloid antibody 25524-1-APDownload protocol
IHC protocol for Beta Amyloid antibody 25524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Neurosci Bull

PMID: 30632006

Authors - Yao Zhang
human,ratWB

Neurotherapeutics

Direct Activation of Protein Phosphatase 2A (PP2A) by Tricyclic Sulfonamides Ameliorates Alzheimer's Disease Pathogenesis in Cell and Animal Models.

Authors - Hui Wei
mouseWB

Int J Neurosci

Activating transcription factor 6 reduces Aβ1-42 and restores memory in Alzheimer's disease model mice.

Authors - Yayun Du
mouseWB

Mol Biol Rep

Ginsenoside compound K ameliorates Alzheimer's disease in HT22 cells by adjusting energy metabolism.

Authors - Xijun Chen
mouseIF

Funct Integr Genomics

A transcriptomic analysis of Nsmce1 overexpression in mouse hippocampal neuronal cell by RNA sequencing.

Authors - Mengting Gong
mouseIF

Exp Cell Res

siRNA knockdown of alanine aminopeptidase impairs myoblast proliferation and differentiation.

Authors - Shion Osana
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

JIMMY (Verified Customer) (04-15-2019)

This antibody detects APP in the brain of 12 months C57BL/6 mice.

  • Applications: Western Blot,