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Annexin A13 Polyclonal antibody

Annexin A13 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25153-1-AP

Synonyms

Annexin 13, annexin A13, Annexin XIII, ANX13, ANXA13, Intestine specific annexin, ISA

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse small intestine tissue using Annexin A13 Polyclonal antibody (25153-1-AP)

    WB analysis of mouse small intestine using 25153-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Annexin A13 Polyclonal antibody (25153-1-AP)

    WB analysis of mouse skeletal muscle using 25153-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using Annexin A13 Polyclonal antibody (25153-1-AP)

    WB analysis of mouse small intestine using 25153-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Annexin A13 Polyclonal antibody (25153-1-AP)

    IHC staining of human small intestine using 25153-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Annexin A13 Polyclonal antibody (25153-1-AP)

    IHC staining of human small intestine using 25153-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse small intestine tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25153-1-AP is Annexin A13 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A13 fusion protein Ag18581
Full Name annexin A13
Calculated Molecular Weight 316 aa, 35 kDa
Observed Molecular Weight 35 kDa / 40 kDa
GenBank Accession NumberBC125158
Gene Symbol ANXA13
Gene ID (NCBI) 312
RRIDAB_2879928
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Annexin A13 antibody 25153-1-APDownload protocol
IHC protocol for Annexin A13 antibody 25153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols