Tested Applications
| Positive WB detected in | mouse small intestine tissue, rat small intestine tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
25153-1-AP targets Annexin A13 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18581 Product name: Recombinant human ANXA13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC125158 Sequence: MGNRHAKASSPQGFDVDRDAKKLNKACKGMGTNEAAIIEILSGRTSDERQQIKQKYKATYGKELEEVLKSELSGNFEKTALALLDHPSEYAARQLQKAMKGLGTDESVLIEVLCTRTNKEIIAIKEAYQRLFDRSLESDVKGDTSGNLKKILVSLLQANRNEGDDVDKDLAGQDAKDLYDAGEGRWGTDELAFNEVLAKRSYKQLRATFQAYQILIGKDIEEAIEEETSGDLQKAYLTLVRCAQDCEDYFAERLYKSMKGAGTDEETLIRIIVTRAEVDLQGIKAKFQEKYQKSLSDMVRSDTSGDFRKLLVALLH Predict reactive species |
| Full Name | annexin A13 |
| Calculated Molecular Weight | 316 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC125158 |
| Gene Symbol | Annexin A13 |
| Gene ID (NCBI) | 312 |
| RRID | AB_2879928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27216 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Annexin A13 (ANXA13) is believed to be the original founder gene of the 12-member vertebrate annexin A family, and it has acquired an intestine-specific expression associated with a highly differentiated intracellular transport function (PMID: 11961095). ANXA13, which is highly expressed in the digestive system and believed to be the original founder gene of the annexin A family (PMID: 28423508). Limited evidence suggests that ANXA13 may play a role in apoptotic cell-mediated immunosuppression, hypertension, and cell death recognition (PMID: 28423508). ANXA13 is highly expressed in the human colon adenocarcinoma cell line HT29.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Annexin A13 antibody 25153-1-AP | Download protocol |
| WB protocol for Annexin A13 antibody 25153-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
