BAALC Polyclonal antibody

BAALC Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24997-1-AP

Synonyms

BAALC



Tested Applications

Positive WB detected inHEK-293 cells, A375 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 24997-1-AP is BAALC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAALC fusion protein Ag21850
Full Name brain and acute leukemia, cytoplasmic
Calculated molecular weight 19 kDa
Observed molecular weight 19-25 kDa
GenBank accession numberBC011517
Gene symbol BAALC
Gene ID (NCBI) 79870
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Brain and acute leukemia cytoplasmic protein, also named as BAALC, is a 180 amino acid protein, which interacts with CAMK2A. BAALC localizes in the cytoplasm and membrane. BAALC is expressed in neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. BAALC may play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.

Protocols

Product Specific Protocols
WB protocol for BAALC antibody 24997-1-APDownload protocol
IHC protocol for BAALC antibody 24997-1-APDownload protocol
IF protocol for BAALC antibody 24997-1-APDownload protocol
IP protocol for BAALC antibody 24997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Chin Med J (Engl)

Expression and prognostic value of brain and acute leukemia, cytoplasmic in meningiomas.

Authors - Yong-Tao He