SODD/BAG4 Antibody

SODD/BAG4 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 13913-1-AP

Synonyms

BAG 4, BAG4, Silencer of death domains, SODD



Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13913-1-AP targets SODD/BAG4 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity Artemia
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SODD/BAG4 fusion protein Ag4905
Full Name BCL2-associated athanogene 4
Calculated molecular weight 457aa,50 kDa
Observed molecular weight 60 kDa, 66-70 kDa
GenBank accession number BC038505
Gene symbol BAG4
Gene ID (NCBI) 9530
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).

Protocols

Product Specific Protocols
WB protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
IF protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
IP protocol for SODD/BAG4 antibody 13913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ArtemiaWB

Gene

Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.

Authors - Mengchen Zhang