Tested Applications
| Positive WB detected in | K-562 cells, MCF-7 cells |
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13913-1-AP targets SODD/BAG4 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, artemia sinica |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4905 Product name: Recombinant human BAG4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 107-457 aa of BC038505 Sequence: ARSRAPYPSTYPVRPELQGQSLNSYTNGAYGPTYPPGPGANTASYSGAYYAPGYTQTSYSTEVPSTYRSSGNSPTPVSRWIYPQQDCQTEAPPLRGQVPGYPPSQNPGMTLPHYPYGDGNRSVPQSGPTVRPQEDAWASPGAYGMGGRYPWPSSAPSAPPGNLYMTESTSPWPSSGSPQSPPSPPVQQPKDSSYPYSQSDQSMNRHNFPCSVHQYESSGTVNNDDSDLLDSQVQYSAEPQLYGNATSDHPNNQDQSSSLPEECVPSDESTPPSIKKIIHVLEKVQYLEQEVEEFVGKKTDKAYWLLEEMLTKELLELDSVETGGQDSVRQARKEAVCKIQAILEKLEKKGL Predict reactive species |
| Full Name | BCL2-associated athanogene 4 |
| Calculated Molecular Weight | 457 aa, 50 kDa |
| Observed Molecular Weight | 60 kDa, 66-70 kDa |
| GenBank Accession Number | BC038505 |
| Gene Symbol | SODD/BAG4 |
| Gene ID (NCBI) | 9530 |
| RRID | AB_2243308 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95429 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SODD/BAG4 antibody 13913-1-AP | Download protocol |
| IP protocol for SODD/BAG4 antibody 13913-1-AP | Download protocol |
| WB protocol for SODD/BAG4 antibody 13913-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gene Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica. | ||
Nat Commun Mettl3-dependent m6A modification attenuates the brain stress response in Drosophila | ||
Mol Med USP14 inhibits mitophagy and promotes tumorigenesis and chemosensitivity through deubiquitinating BAG4 in microsatellite instability-high colorectal cancer |
