Tested Applications
| Positive WB detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22964-1-AP targets BARD1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18965 Product name: Recombinant human BARD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 428-777 aa of BC126426 Sequence: GETLLHIASIKGDIPSVEYLLQNGSDPNVKDHAGWTPLHEACNHGHLKVVELLLQHKALVNTTGYQNDSPLHDAAKNGHVDIVKLLLSYGASRNAVNIFGLRPVDYTDDESMKSLLLLPEKNESSSASHCSVMNTGQRRDGPLVLIGSGLSSEQQKMLSELAVILKAKKYTEFDSTVTHVVVPGDAVQSTLKCMLGILNGCWILKFEWVKACLRRKVCEQEEKYEIPEGPRRSRLNREQLLPKLFDGCYFYLWGTFKHHPKDNLIKLVTAGGGQILSRKPKPDSDVTQTINTVAYHARPDSDQRFCTQYIIYEDLCNYHPERVRQGKVWKAPSSWFIDCVMSFELLPLDS Predict reactive species |
| Full Name | BRCA1 associated RING domain 1 |
| Calculated Molecular Weight | 777 aa, 87 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC126426 |
| Gene Symbol | BARD1 |
| Gene ID (NCBI) | 580 |
| RRID | AB_2879190 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q99728 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BARD1 (BRCA1-associated RING domain protein 1) is a multifunctional protein that interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. Moreover, BARD1 is central to DNA repair, tumor suppression, and cancer predisposition.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BARD1 antibody 22964-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BARD1 Polyclonal antibody (Cat# 22964-1-AP) has 10 citations published in journals including Metabolism, Advanced Science, Cell Death & Disease, PLoS Biology, American Journal of Physiology, Molecules, BBA Molecular Basis of Disease, Discovery Oncology, and eLife. Research fields span lipid metabolism, cancer stem cell formation, non-small cell lung cancer, ovarian cancer, homologous recombination repair, BRCA1 stabilization, triple-negative breast cancer, hepatocellular carcinoma, and replication restart mechanisms.
| Species | Application | Title |
|---|---|---|
Metabolism Natural polyketide enterocin inhibits ASGR1 to enhance cholesterol efflux and regulate hepatic lipid metabolism. | ||
Adv Sci (Weinh) CHB-Induced Immune Zonation Chaos Elicited LXRα-mediated Lipid Metabolism Disorders in Kupffer Cells to Induce Cancer Stem Cell Formation. | ||
Cell Death Dis Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis | ||
Cell Death Dis CBX2 phase-separation contributes to homologous recombination repair and drug resistance in ovarian cancer. | ||
PLoS Biol The deubiquitinating enzyme Cezanne stabilizes BRCA1 by counteracting APC/C and Ube2S-dependent Lys11-linked ubiquitination. | ||
Am J Physiol Cell Physiol Synergistic Effects of Olaparib and Palbociclib in Resistant epithelial ovarian cancer |
Reviews
What customers say
The single review for 22964-1-AP is from Vikas Maloji Rao (April 2025), who used the antibody for Western Blot on HEK-293 cells at a 1:1000 dilution. He rated it 5 stars and commented that it "worked very well" and is "highly recommended." Overall, the feedback is very positive, indicating strong performance and reliability in his application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vikas (Verified Customer) (04-08-2025) | worked very well. Highly recommended.
|
